Literature DB >> 2392881

The synthesis of influenza virus negative-strand RNA takes place in insoluble complexes present in the nuclear matrix fraction.

J A López-Turiso1, C Martínez, T Tanaka, J Ortín.   

Abstract

The replication of influenza virus RNA in vitro has been studied by cell fractionation of MDCK-infected cells and characterization of in vitro synthesized RNA. Analysis of the RNA product polarity by liquid hybridization to excess single-stranded DNA probes shows that only the RNP complexes present in the nuclear matrix fraction are able to synthesize negative-polarity RNA. This RNA product has been characterized as authentic vRNA by size analysis, RNase-protection by unlabelled, positive-polarity riboprobes and T1-fingerprinting. Priming the in vitro reaction with ApG stimulates preferentially the synthesis of positive-polarity RNA, while ApGpU stimulates both positive and negative-polarity RNA synthesis.

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Year:  1990        PMID： 2392881     DOI： 10.1016/0168-1702(90)90056-h

Source DB:  PubMed          Journal:  Virus Res        ISSN： 0168-1702            Impact factor:   3.303


  21 in total

1.  Role of the influenza virus M1 protein in nuclear export of viral ribonucleoproteins.

Authors:  M Bui; E G Wills; A Helenius; G R Whittaker
Journal:  J Virol       Date:  2000-02       Impact factor: 5.103

2.  The PB1 subunit alone can catalyze cRNA synthesis, and the PA subunit in addition to the PB1 subunit is required for viral RNA synthesis in replication of the influenza virus genome.

Authors:  Y Nakagawa; K Oda; S Nakada
Journal:  J Virol       Date:  1996-09       Impact factor: 5.103

3.  Regulation of influenza virus RNA polymerase activity by cellular and viral factors.

Authors:  K Shimizu; H Handa; S Nakada; K Nagata
Journal:  Nucleic Acids Res       Date:  1994-11-25       Impact factor: 16.971

4.  Individual expression of influenza virus PA protein induces degradation of coexpressed proteins.

Authors:  J J Sanz-Ezquerro; S de la Luna; J Ortín; A Nieto
Journal:  J Virol       Date:  1995-04       Impact factor: 5.103

5.  The amino-terminal one-third of the influenza virus PA protein is responsible for the induction of proteolysis.

Authors:  J J Sanz-Ezquerro; T Zürcher; S de la Luna; J Ortín; A Nieto
Journal:  J Virol       Date:  1996-03       Impact factor: 5.103

6.  Mutational analysis identifies functional domains in the influenza A virus PB2 polymerase subunit.

Authors:  B Perales; S de la Luna; I Palacios; J Ortín
Journal:  J Virol       Date:  1996-03       Impact factor: 5.103

7.  The host protein CLUH participates in the subnuclear transport of influenza virus ribonucleoprotein complexes.

Authors:  Tomomi Ando; Seiya Yamayoshi; Yuriko Tomita; Shinji Watanabe; Tokiko Watanabe; Yoshihiro Kawaoka
Journal:  Nat Microbiol       Date:  2016-05-16       Impact factor: 17.745

Review 8.  Snapshots: chromatin control of viral infection.

Authors:  David M Knipe; Paul M Lieberman; Jae U Jung; Alison A McBride; Kevin V Morris; Melanie Ott; David Margolis; Amelia Nieto; Michael Nevels; Robin J Parks; Thomas M Kristie
Journal:  Virology       Date:  2013-01-05       Impact factor: 3.616

9.  Basic residues of the helix six domain of influenza virus M1 involved in nuclear translocation of M1 can be replaced by PTAP and YPDL late assembly domain motifs.

Authors:  Eric Ka-Wai Hui; Subrata Barman; Tae Yong Yang; Debi P Nayak
Journal:  J Virol       Date:  2003-06       Impact factor: 5.103

10.  Genetic trans-complementation establishes a new model for influenza virus RNA transcription and replication.

Authors:  Núria Jorba; Rocío Coloma; Juan Ortín
Journal:  PLoS Pathog       Date:  2009-05-29       Impact factor: 6.823
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