Antitumour activity of abiraterone and diethylstilboestrol when administered sequentially to men with castration-resistant prostate cancer.

BACKGROUND: Abiraterone is a standard treatment for men with castration-resistant prostate cancer (CRPC). We evaluated the antitumour activity of abiraterone following the synthetic oestrogen diethylstilboestrol (DES).
METHODS: Castration-resistant prostate cancer patients treated with abiraterone were identified. Demographics, response variables and survival data were recorded.
RESULTS: Two-hundred and seventy-four patients received abiraterone, 114 (41.6%) after DES. Pre-chemotherapy abiraterone resulted in ≥50% PSA declines in 35/41 (85.4%) DES-naïve and 20/27 (74.1%) DES-treated patients. Post-docetaxel abiraterone resulted in ≥50% PSA declines in 40/113 (35.4%) DES-naïve and 23/81 (28.4%) DES-treated patients. Time to PSA progression was similar regardless of prior DES.
CONCLUSION: Abiraterone has important antitumour activity in men with CRPC even after DES exposure.

In 1941, oestrogen therapy became the first reported hormonal manipulation for men with advanced prostate cancer (Huggins and Hodges, 2002), but was superseded by surgical and medical castration (Scott and Benjamin, 1945; Parmar ). Diethylstilboestrol (DES) is a synthetic non-steroidal oestrogen that has been widely used in men with castration-resistant prostate cancer (CRPC). Several mechanisms have been proposed to explain DES activity, including reduction of luteinizing hormone, testosterone and androgenic steroid levels (Bosset ), inhibition of telomerase activity (Geier ); direct binding of the androgen receptor (AR) (Wang ) and suppression of β-tubulin isotypes (Montgomery ). High-dose (5 mg daily) DES was associated with cardiovascular toxicity (Malkowicz, 2001), but low-dose DES 1 mg daily had a more acceptable therapeutic ratio, with reported activity including ⩾50% PSA declines in 23–43% of patients (Smith ; Manikandan ; Clemons ; Wilkins ) and time to PSA progression of 4–4.6 months (Clemons ; Wilkins ). Combined with dexamethasone, DES treatment resulted in ⩾50% PSA declines in 64–68% of patients in a small randomised study (Shamash ), but venothromboembolic events occurred in 22% of patients in the combination arm. Diethylstilboestrol continues to be used in men with CRPC in several countries. Furthermore, a clinical trial randomising patients between LHRH analogue treatment or transcutaneous oestrogen patches is currently in progress (PATCH trial, NCT00303784).

Abiraterone is an irreversible, selective, CYP17A1 inhibitor that blocks steroidal conversion to androgens and oestrogens. Large Phase III studies confirmed abiraterone as an effective treatment in CRPC (de Bono ; Ryan ). With six survival-prolonging treatments for CRPC, understanding the activity of treatments administered sequentially has become critically important for physicians. This is particularly relevant given the current financial pressures on healthcare budgets and the hypothesis that sequential therapies may impact the efficacy of later-line treatments acting on similar pathways of tumour growth. We therefore sought to evaluate the antitumour activity of abiraterone after DES therapy.

Patients and Methods

This was a retrospective analysis of all patients treated with abiraterone at the Royal Marsden NHS Foundation Trust (RM) hospital in Chelsea, London and Sutton, Surrey, UK. Patients were identified from the pharmacy dispensing records and received abiraterone with concomitant prednisolone 10 mg daily or dexamethasone 0.5 mg daily in the settings of Phase I, II or III clinical trials reported previously (Attard ; Carden ; Attard ; Reid ; de Bono ; Ryan ) (N=123) and expanded access programs or post approval (N=151). All clinical trial patients underwent baseline and three-monthly imaging by computed tomography (CT) and bone scan. Patients treated outside of trials had CT and bone scan imaging at baseline and every 6 months as per local practice guidelines.

Demographic and treatment-related data were collected using the electronic hospital record. The objective of this analysis was to report the response rate and survival of patients treated with abiraterone, taking into account prior treatment with DES and docetaxel. This analysis was approved by the RM Clinical Audit Committee (SE143). Prostate-specific antigen and soft tissue responses were assessed using Prostate Cancer Working Group 2 criteria (Scher ). Prostate-specific antigen progression was defined as a confirmed ⩾25% rise from nadir in patients with a ⩾50% PSA decline and as a confirmed ⩾25% rise from baseline in the remaining patients, disregarding progression prior to 12 weeks of therapy. Due to the impact of patient censoring, time to PSA progression in the post-docetaxel groups was calculated using bootstrap analysis. Radiographic responses and progression were assessed using RECIST 1.1 in patients with measurable soft tissue disease (Eisenhauer ). Date cut-off for follow-up was 31 December 2012 and patients still alive or lost to follow-up were censored.

Descriptive statistics and Kaplan–Meier survival analyses were performed using IBM SPSS Statistics v20 (IBM).

Results

Patient characteristics

Between January 2006 and October 2012, a total of 274 patients received abiraterone. The patient population is described in Table 1. Of these, 114 were treated with DES prior to abiraterone at doses of 1 to 3 mg per day with or without aspirin and/or dexamethasone as per institutional standards. The majority of patients (106, 96.3%, 4 patients not known) received at least 30 days of DES. Diethylstilboestrol was discontinued in 86 (75.4%) men due to progressive disease, whereas 18 (15.8%) discontinued due to toxicity (reason unknown in 10 patients). A total of 68 patients were treated with abiraterone prior to chemotherapy, whereas 206 patients were treated with abiraterone after docetaxel (see Table 2). The median follow-up for the overall cohort was 58 months. The antitumour activity of abiraterone is lower in docetaxel-treated compared with docetaxel-naïve patients (Attard ; Reid ; Ryan ; de Bono ; Ryan et al, 2013), and we have therefore reported the activity of abiraterone post-DES in these two settings separately. Of the 68 docetaxel-naïve patients treated with abiraterone, 27 had previously received DES. Of the 206 docetaxel-treated patients who received abiraterone, 87 had been treated with DES.

Table 1

Patient characteristics

	AA pre-docetaxel DES pre-treated (N=27)	AA pre-docetaxel DES naïve (N=41)	AA post-docetaxel DES pre-treated (N=87)	AA post-docetaxel DES naïve (N=119)
Median age at diagnosis (years)	63.2	64.1	63.1	62.5
Range	45.3–80.7	44.8–79.6	43.9–77.7	41.8–77.3
Median Gleason score	8	8	7	8
Range	6–10	6–10	2–10	4–10
Median time from diagnosis to CRPC (years)	3.3	3.6	3.8	2.3
Range	0.3–13.7	0.6– 16.2	0.7–14.3	0.1–14.6
Treatments prior to DES (or abiraterone), N (%)
Antiandrogens	24 (89)	40 (98)	83 (95)	116 (97)
Single-agent steroids	5 (19)	13 (32)	12 (14)	53 (45)
DES administered with steroids, N (%)	5 (19)	NA	21 (24)	NA
Docetaxel prior to DES, N (%)	NA	NA	25 (29)	NA
ECOG performance status at AA, N (%)			(NA: 3)	(NA: 3)
0	15 (56)	25 (61)	18 (21)	25 (21)
1	12 (44)	16 (39)	52 (60)	74 (62)
2			14 (16)	17 (14)
Metastases at AA, N (%)
Bone	22 (81)	33 (80)	81 (93)	107 (90)
Nodal	9 (33)	18 (44)	38 (44)	59 (50)
Visceral	5 (19)	2 (5)	13 (15)	20 (17)
Median PSA at AA (μg l−1)	193	39.9	435	213
Range	14.8–2642	3.5–870	10–8580	2.3–10 335
Median haemoglobin at AA (g l−1)	12.7	12.7	11.5	11.2
Range	9.7–15.4	10.0–15.1	8.1–14.7	8.0–16.5
Median alkaline phosphatase at AA (U l−1)	87	96	158	147
Range	41–518	43–642	23–2044	34–2163
Median albumin at AA (g l−1)	36	37	34	35
Range	27–42	31–48	23–41	24–47
Median lactate dehydrogenase at AA (U l−1)	172	174	205	217
Range	78–576	110 –298	72–2490	119–1659

Abbreviations: AA=Abiraterone acetate; CRPC=castration-resistant prostate cancer; DES=diethylstilboestrol; ECOG=Eastern Cooperative Oncology Group; PSA=prostate-specific antigen.

Table 2

Activity of abiraterone grouped by DES exposure

AA pre-docetaxel	DES pre-treated (N=27)	DES naïve (N=41)
Median duration of AA treatment (months, m)	11.4	16.6
95% CI	7.6–16.8	12–21.7
Median duration of DES treatment (m)	11.4
95% CI	8–15
	N(%)	N(%)
⩾50% PSA decline	20 (74.1)	35 (85.4)
⩾30% PSA decline	22 (81.5)	37 (90.2)
Soft tissue response	4/10 (40)	9/18 (50)
Median survival from start of AA (m)	36.7	40.5
95% CI	11.1–62.4	27.8–53.2
Median time to PSA progression on AA (m)	8.5	9.2
95% CI	3.0–14.1	5.7–12.7
Number of evaluable patients	24	39
Median time to radiographic soft tissue progression on AA (m)	11.9	13.8
95% CI	0–30.6	8.3–19.3
Number of evaluable patients	10	18
Abiraterone (AA) post-docetaxel	DES pre-treated (N=87)	DES naïve (N=119)
Median duration of AA treatment (m)	5.5	4.3
95% CI	4.4–6.5	3.7–5.6
Median duration of DES treatment (m)	9
95% CI	5.8–15
	N(%)	N(%)
⩾50% PSA decline	23/81 (28.4)	40/113 (35.4)
⩾30% PSA decline	34/81 (42)	55/113 (48.7)
Soft tissue response	9/36 (25)	6/40 (15)
Median survival from start of AA (m)	13.4	13.4
95% CI	9.8–17	9.8–16.7
Median time to PSA progression on AA (m)	4.3	4.3
95% CI	3.5 –5.5	3.7–5.5
Number of evaluable patients	71	91
Median time to radiographic soft tissue progression on AA (m)	2.9	2.7
95% CI	1.6–4.2	2.5–2.9
Number of evaluable patients	36	40
DES post abiraterone, N=31
Performance status at start of DES, N (%)
0	4 (13)
1	10 (32)
2	13 (42)
3	4 (13)
Median PSA at start of DES (μg l−1)	399
Range	13.8–22'299
	DES (PSA evaluable N=23)	Prior abiraterone
Median time on treatment (m)	2.7	11.2
95% CI	1.8– 4.4
Range	0.1–31.2	0.9–32.7
⩾50% PSA decline, N (%)	2/23 (9)	17/31 (55)
Median survival from start of DES (m)	4.8
Range	0.2–46.4

Abbreviations: AA=abiraterone acetate; CI=confidence interval; DES=diethylstilboestrol; PSA=prostate-specific antigen.

Abiraterone administered prior to docetaxel

Activity measures for the 27 men treated with abiraterone following DES are shown in Table 2 and Figure 1, including ⩾50% PSA declines in 20 patients (74.1%), a median time to PSA progression of 8.5 months and median duration of treatment of 11.4 months. Median survival from start of abiraterone was 36.7 months (95% CI 11.1–62.4). In comparison, ⩾50% PSA declines occurred in 35 of the 41 patients (85.4%) who received abiraterone without prior DES exposure, with median time to PSA progression of 9.2 months and median treatment duration of 16.6 months. In these patients, the median survival from start of abiraterone was 40.5 months (95% CI 27.8–53.2).

Figure 1

Waterfall plots of maximum PSA decline on abiraterone. PSA increases of >50% were capped. Abbreviation: Pts=patients.

Abiraterone administered after docetaxel

A total of 87 men were treated with abiraterone after DES and docetaxel. Maximum PSA declines of ⩾50% occurred in 23 of the 81 evaluable patients (28.4%) and median time to PSA progression was 4.3 months (16 patients discontinued therapy prior to 12 weeks and were not included in progression analysis). Patients remained on treatment with abiraterone for a median of 5.5 months. Median survival was 13.4 months (95% CI 9.8–17.0). A total of 119 patients received abiraterone after docetaxel with no prior DES exposure. Declines of PSA ⩾50% occurred in 40 of 113 patients evaluable for PSA response (35.4%) with a median time to PSA progression of 4.3 months (28 patients discontinued therapy prior to 12 weeks). Patients received abiraterone for a median of 4.3 months and the median survival was 13.4 months (95% CI 9.8–16.7).

Diethylstilboestrol administered after abiraterone

A total of 31 patients received DES after abiraterone. In this cohort, 28 (90.3%) patients had also received docetaxel and one patient had been treated with cabazitaxel chemotherapy. The median duration of abiraterone prior to DES was 11.2 months (see Table 2). The median duration of DES treatment was 2.7 months (range 0.1–31.2). Diethylstilboestrol was discontinued due to progression in 18 (62%) and toxicity in four (13.8%) patients (reason unknown in five patients and patient death in three patients). Prostate-specific antigen declines of ⩾50% occurred in 8.7% (2/23) evaluable patients. No objective soft tissue responses were seen in the six evaluable patients. The majority of patients did not have follow-up scans due to declining performance status.

Of 14 patients who received DES for >3 months, four were docetaxel-naïve at start of DES treatment (three of these patients, however, received docetaxel after DES) and seven (50%) had ⩾90% PSA declines on abiraterone.

Another patient discontinued abiraterone after 27 days of treatment due to an acute pulmonary embolism; abiraterone was not re-started because it was impossible to exclude a causal relationship. This patient had a subsequent 80% PSA decline on DES and remained on treatment for 31.3 months.

Discussion

In this large, single-centre cohort of CRPC patients, abiraterone retained significant and clinically important activity after DES treatment. Prior DES exposure appeared to have minimal impact on overall abiraterone activity. We also report the activity of DES after abiraterone, which was prospectively collected for our patients. In these patients, DES was primarily used in the end-stage setting when no other treatment was possible due to availability or patient fitness and its activity in this setting was very modest. Patients who received >3 months DES treatment were mainly patients who had substantial PSA declines and long durations of treatment on abiraterone or those who stopped abiraterone prematurely. The short treatment duration and survival on DES after abiraterone and docetaxel reflect the advanced state of these patients and may therefore have underestimated the activity of DES post abiraterone. Recent reports suggest both abiraterone and enzalutamide have lower activity in patients with poorer performance status (de Bono ; Scher ; Loriot ; Noonan ). We did not have robust data available to evaluate the activity of DES treatment prior to abiraterone.

These data are retrospective in nature. The sample size of the cohorts, especially in chemotherapy-naïve patients, is limited. Furthermore, we assessed abiraterone activity using PSA endpoints, which are commonly used activity endpoints, but not robust surrogates of overall survival(Fleming ). Of note, the median time on DES in our pre-chemotherapy cohort was considerably longer than previously reported (Wilkins ). It is likely that patients with a good response to prior hormonal manipulations and no clinical indication for cytotoxic chemotherapy were positively selected in the Phase II trials of abiraterone pre-chemotherapy.

The landscape of treatments for CRPC is changing rapidly and abiraterone was recently approved in the pre-chemotherapy setting. This analysis demonstrates that abiraterone remains an active treatment in patients after prior DES treatment. Given the efficacy of abiraterone and the toxicity of DES, abiraterone will increasingly be used prior to DES, although financial constraints may limit the use of abiraterone in certain settings. The antitumour activity of DES after abiraterone in this cohort of patients was limited, indicating that other treatment options or clinical trials should be considered in fit abiraterone-resistant patients. Similarly, our data suggest very limited activity with DES in poor performance, end-stage patients who previously received abiraterone.