Literature DB >> 23928044

Rapid parallel flow cytometry assays of active GTPases using effector beads.

Tione Buranda1, Soumik BasuRay, Scarlett Swanson, Jacob Agola, Virginie Bondu, Angela Wandinger-Ness.   

Abstract

We describe a rapid assay for measuring the cellular activity of small guanine triphosphatases (GTPases) in response to a specific stimulus. Effector-functionalized beads are used to quantify in parallel multiple GTP-bound GTPases in the same cell lysate by flow cytometry. In a biologically relevant example, five different Ras family GTPases are shown for the first time to be involved in a concerted signaling cascade downstream of receptor ligation by Sin Nombre hantavirus.
Copyright © 2013 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Cdc42; Hantavirus; Integrins; Rab GTPases; Rac; Rho

Mesh：

Substances：

Year:  2013        PMID： 23928044      PMCID： PMC3848308          DOI： 10.1016/j.ab.2013.07.039

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  47 in total

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Review 4.  Rho and Rac take center stage.

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Authors:  A J Ridley; A Hall
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8.  Quantitative model of antibody- and soluble CD4-mediated neutralization of primary isolates and T-cell line-adapted strains of human immunodeficiency virus type 1.

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9.  Integrins regulate Rac targeting by internalization of membrane domains.

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  14 in total

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3.  Quantitative bead-based flow cytometry for assaying Rab7 GTPase interaction with the Rab-interacting lysosomal protein (RILP) effector protein.

Authors:  Jacob O Agola; Daniel Sivalingam; Daniel F Cimino; Peter C Simons; Tione Buranda; Larry A Sklar; Angela Wandinger-Ness
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5.  Elevated cytokines, thrombin and PAI-1 in severe HCPS patients due to Sin Nombre virus.

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6.  Equilibrium and kinetics of Sin Nombre hantavirus binding at DAF/CD55 functionalized bead surfaces.

Authors:  Tione Buranda; Scarlett Swanson; Virginie Bondu; Leah Schaefer; James Maclean; Zhenzhen Mo; Keith Wycoff; Archana Belle; Brian Hjelle
Journal:  Viruses       Date:  2014-03-10       Impact factor: 5.048

Review 7.  Hantavirus Gn and Gc envelope glycoproteins: key structural units for virus cell entry and virus assembly.

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