BACKGROUND: Nosocomial transmission of Candida spp. has not been fully explored and previous studies have shown conflicting results. AIM: To evaluate the possible nosocomial transmission of Candida spp. on an intensive care unit (ICU). METHODS: A prospective study was conducted for a period of 19 months, including all patients on our ICU with growth of Candida spp. from surveillance and directed cultures. Molecular typing with repetitive sequence-based polymerase chain reaction was used to define genotype relationships between the Candida albicans and Candida glabrata isolates. Candida isolates obtained from blood cultures taken from patients in our county outside the ICU were used as a reference. Temporal cluster analysis was performed to evaluate genotype distribution over time. FINDINGS: Seventy-seven patients with 78 ICU stays, representing 12% of all ICU stays, were found to harbour 180 isolates of Candida spp. Molecular typing revealed 27 C. albicans genotypes and 10 of C. glabrata. Possible clustering, indicated by overlapping stays of patients with indistinguishable candida genotypes, was observed on seven occasions with C. albicans and on two occasions with C. glabrata. Two C. albicans genotypes were found significantly more often in the ICU group compared with the reference group. Moreover, C. albicans genotypes isolated from more than one patient were significantly more often found in the ICU group. Temporal cluster analysis revealed a significantly increased number of pairs with indistinguishable genotypes at a 21-day interval, indicating clustering. CONCLUSION: This study indicates possible transmission of C. albicans between ICU patients based on genotyping and temporal cluster analysis.
BACKGROUND: Nosocomial transmission of Candida spp. has not been fully explored and previous studies have shown conflicting results. AIM: To evaluate the possible nosocomial transmission of Candida spp. on an intensive care unit (ICU). METHODS: A prospective study was conducted for a period of 19 months, including all patients on our ICU with growth of Candida spp. from surveillance and directed cultures. Molecular typing with repetitive sequence-based polymerase chain reaction was used to define genotype relationships between the Candida albicans and Candida glabrata isolates. Candida isolates obtained from blood cultures taken from patients in our county outside the ICU were used as a reference. Temporal cluster analysis was performed to evaluate genotype distribution over time. FINDINGS: Seventy-seven patients with 78 ICU stays, representing 12% of all ICU stays, were found to harbour 180 isolates of Candida spp. Molecular typing revealed 27 C. albicans genotypes and 10 of C. glabrata. Possible clustering, indicated by overlapping stays of patients with indistinguishable candida genotypes, was observed on seven occasions with C. albicans and on two occasions with C. glabrata. Two C. albicans genotypes were found significantly more often in the ICU group compared with the reference group. Moreover, C. albicans genotypes isolated from more than one patient were significantly more often found in the ICU group. Temporal cluster analysis revealed a significantly increased number of pairs with indistinguishable genotypes at a 21-day interval, indicating clustering. CONCLUSION: This study indicates possible transmission of C. albicans between ICU patients based on genotyping and temporal cluster analysis.
Authors: David M Jacobs; Nicholas D Beyda; Orarik Asuphon; M Jahangir Alam; Kevin W Garey Journal: Mycopathologia Date: 2014-08-31 Impact factor: 2.574
Authors: L S Damasceno; T M J S Leitão; M L Taylor; M M Muniz; R M Zancopé-Oliveira Journal: Eur J Clin Microbiol Infect Dis Date: 2015-11-20 Impact factor: 3.267
Authors: Angela Ahlquist Cleveland; Lee H Harrison; Monica M Farley; Rosemary Hollick; Betsy Stein; Tom M Chiller; Shawn R Lockhart; Benjamin J Park Journal: PLoS One Date: 2015-03-30 Impact factor: 3.240
Authors: Jesús Guinea; Maiken C Arendrup; Rafael Cantón; Emilia Cantón; Julio García-Rodríguez; Ana Gómez; Elia Gómez G de la Pedrosa; Rasmus K Hare; Beatriz Orden; Maurizio Sanguinetti; Javier Pemán; Brunella Posteraro; Alba Ruiz-Gaitán; Gabriella Parisi; Daniel Archimedes Da Matta; Arnaldo L Colombo; Carlos Sánchez-Carrillo; Elena Reigadas; Patricia Muñoz; Pilar Escribano Journal: Front Cell Infect Microbiol Date: 2020-05-05 Impact factor: 5.293
Authors: Yan Wang; Ying Zhang; Treasure M McGuire; Samantha A Hollingworth; Mieke L Van Driel; Lu Cao; Xue Wang; Yalin Dong Journal: Front Pharmacol Date: 2021-03-22 Impact factor: 5.810