| Literature DB >> 23922255 |
Abu Kausar1, Catherine J Mitran, Yimeng Li, Julianne M Gibbs-Davis.
Abstract
You spin me round: Using a destabilizing abasic site and high concentration of ligase, rapid DNA self-replication in an isothermal ligase chain reaction (LCR) was produced. Both destabilization and rapid ligation are essential for proper LCR replication. This method also provides insight into prebiotic nucleotide replication and is a potential amplification method for biodiagnostics.Keywords: DNA replication; autonomous replication; isothermal DNA amplification; oligonucleotides; origins of life
Mesh：
Substances：
Year: 2013 PMID： 23922255 DOI： 10.1002/anie.201303225
Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336