Literature DB >> 23917316

SbcCD-mediated processing of covalent gyrase-DNA complex in Escherichia coli.

Sandra Aedo1, Yuk-Ching Tse-Dinh.   

Abstract

Quinolones trap the covalent gyrase-DNA complex in Escherichia coli, leading to cell death. Processing activities for trapped covalent complex have not been characterized. A mutant strain lacking SbcCD nuclease activity was examined for both accumulation of gyrase-DNA complex and viability after quinolone treatment. Higher complex levels were found in ΔsbcCD cells than in wild-type cells after incubation with nalidixic acid and ciprofloxacin. However, SbcCD activity protected cells against the bactericidal action of nalidixic acid but not ciprofloxacin.

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Year:  2013        PMID: 23917316      PMCID: PMC3811449          DOI: 10.1128/AAC.00130-13

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN: 0066-4804            Impact factor:   5.191


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