| Literature DB >> 23913294 |
Aziz El Hage1, Jonathan Houseley.
Abstract
Pulsed-field gel electrophoresis (PFGE) is a technique that resolves chromosome-sized DNA molecules in an agarose gel. As well as DNA mapping and karyotyping applications, PFGE techniques are well adapted to follow DNA rearrangements over time in a quantitative manner. Because of the very large sizes of the DNA molecules analyzed, DNA preparation, electrophoresis, and Southern blotting processes present unique challenges in PFGE experiments. In this chapter, we describe a robust PFGE protocol covering the preparation of intact Saccharomyces cerevisiae chromosomal DNA, specific running conditions for the resolution of small, medium- and large-sized chromosomes and their by-products, and basic Southern blotting and hybridization instructions for the analysis of these molecules.Entities:
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Year: 2013 PMID： 23913294 DOI： 10.1007/978-1-62703-565-1_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745