Detailed analysis of promoter-associated RNA.

The detailed analysis of noncoding RNA is an upcoming necessity due to a plethora of recently identified components of this class of molecules. The investigation of their structure, directionality, intracellular localization, interaction with other cellular elements is useful to understand the role they play in transcriptional regulation. In this chapter, we describe some techniques, meant to determine very important features of promoter-associated RNAs, in order to clarify their functionality.