| Literature DB >> 23913155 |
Abstract
This chapter describes an LC-ESI-MS method for the DAR and drug load distribution analysis that is suitable for lysine-linked ADCs. The ADC sample is desalted using a reversed-phase LC column with an acetonitrile gradient prior to online MS analysis. The MS spectrum is processed (deconvoluted) and converted to a series of zero charge state masses that corresponds to the increasing number of drugs in the ADC. Integration of the mass peak area allows the calculation of the DAR and drug load distribution of ADCs.Entities:
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Year: 2013 PMID： 23913155 DOI： 10.1007/978-1-62703-541-5_18
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745