Literature DB >> 23913153

Drug-to-antibody ratio (DAR) by UV/Vis spectroscopy.

Yan Chen1.   

Abstract

UV/Vis spectroscopy is a simple and convenient method to determine protein concentrations as well as the average number of drugs that are conjugated to the antibody in an antibody-drug conjugate (ADC). Using the measured absorbances of the ADC and the extinction coefficients of the antibody and the drug, the average drug-to-antibody ratio (DAR) can be determined.

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Year:  2013        PMID： 23913153     DOI： 10.1007/978-1-62703-541-5_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

1.  Antibody-Mediated Endocytosis of Polysialic Acid Enables Intracellular Delivery and Cytotoxicity of a Glycan-Directed Antibody-Drug Conjugate.

Authors:  Emily C Cox; Dana N Thornlow; Michaela A Jones; Jordan L Fuller; Judith H Merritt; Matthew J Paszek; Christopher A Alabi; Matthew P DeLisa
Journal:  Cancer Res       Date:  2019-02-26       Impact factor: 12.701

Review 2.  Challenges and new frontiers in analytical characterization of antibody-drug conjugates.

Authors:  Anil Wagh; Hangtian Song; Ming Zeng; Li Tao; Tapan K Das
Journal:  MAbs       Date:  2018-01-05       Impact factor: 5.857

3.  Native mass spectrometry and ion mobility characterization of trastuzumab emtansine, a lysine-linked antibody drug conjugate.

Authors:  Julien Marcoux; Thierry Champion; Olivier Colas; Elsa Wagner-Rousset; Nathalie Corvaïa; Alain Van Dorsselaer; Alain Beck; Sarah Cianférani
Journal:  Protein Sci       Date:  2015-03-31       Impact factor: 6.725

4.  Therapeutic efficacy of antibody-drug conjugates targeting GD2-positive tumors.

Authors:  Daniel V Kalinovsky; Alexey V Kibardin; Irina V Kholodenko; Elena V Svirshchevskaya; Igor I Doronin; Mariya V Konovalova; Maria V Grechikhina; Fedor N Rozov; Sergey S Larin; Sergey M Deyev; Roman V Kholodenko
Journal:  J Immunother Cancer       Date:  2022-06       Impact factor: 12.469

5.  The cytotoxic conjugate of highly internalizing tetravalent antibody for targeting FGFR1-overproducing cancer cells.

Authors:  Marta Poźniak; Natalia Porębska; Mateusz Adam Krzyścik; Aleksandra Sokołowska-Wędzina; Kamil Jastrzębski; Martyna Sochacka; Jakub Szymczyk; Małgorzata Zakrzewska; Jacek Otlewski; Łukasz Opaliński
Journal:  Mol Med       Date:  2021-05-07       Impact factor: 6.354

6.  Efficient Labeling of Native Human IgG by Proximity-Based Sortase-Mediated Isopeptide Ligation.

Authors:  Wendy Yu; Kevin P Gillespie; Bonirath Chhay; Anne-Sophie Svensson; Per-Åke Nygren; Ian A Blair; Feifan Yu; Andrew Tsourkas
Journal:  Bioconjug Chem       Date:  2021-05-24       Impact factor: 6.069

7.  Real-Time Analysis on Drug-Antibody Ratio of Antibody-Drug Conjugates for Synthesis, Process Optimization, and Quality Control.

Authors:  Yubo Tang; Feng Tang; Yang Yang; Lei Zhao; Hu Zhou; Jinhua Dong; Wei Huang
Journal:  Sci Rep       Date:  2017-08-10       Impact factor: 4.379

8.  A Rationally Designed ICAM1 Antibody Drug Conjugate for Pancreatic Cancer.

Authors:  Jing Huang; Agoston T Agoston; Peng Guo; Marsha A Moses
Journal:  Adv Sci (Weinh)       Date:  2020-11-03       Impact factor: 16.806

Review 9.  Antibody-Drug Conjugates for Cancer Therapy: Chemistry to Clinical Implications.

Authors:  Nirnoy Dan; Saini Setua; Vivek K Kashyap; Sheema Khan; Meena Jaggi; Murali M Yallapu; Subhash C Chauhan
Journal:  Pharmaceuticals (Basel)       Date:  2018-04-09

Review 10.  The Use of Antibody-Antibiotic Conjugates to Fight Bacterial Infections.

Authors:  Marco Cavaco; Miguel A R B Castanho; Vera Neves
Journal:  Front Microbiol       Date:  2022-03-07       Impact factor: 5.640
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