Yun-Kang Yang1, Xiao-Dong Peng, Yong-Hong Li, Zheng-Rong Wang, Huang Chang-quan, Wang Hui, Qing-Xiu Liu. 1. From the *Key Laboratory of Chronobiology of Health Ministry, Basic and Forensic School, Sichuan University, Chengdu; †Department of Oncology, The Affiliated Hospital of North Sichuan Medical College, Nanchong; and ‡Key Laboratory of Biological Effect of Physical Field and Instrument of Sichuan Provincial Department of Education and §Department of Electronic Engineering, Chengdu University of Information Technology, Chengdu, Sichuan; and ∥Department of Geriatrics, The Third Hospital of Mian Yang, Mian Yang, China.
Abstract
OBJECTIVES: In the present study, it was examined whether polymorphism of circadian locomotor output cycle kaput (CLOCK) gene 3111T/C was associated with susceptibility of Alzheimer disease (AD). METHODS: This study was conducted using a case-control method. Genotypes of apolipoprotein E (APOE e4) and CLOCK gene 3111T/C were determined by restriction fragment length polymorphism method and TaqMan assay method, respectively. RESULTS: In this study, we gathered 296 unrelated AD patients and 423 control subjects. Both in the whole sample and APOE e4 noncarriers, prevalence of C carriers in CLOCK gene 3111T/C in AD patients was significantly higher than that in control subjects (in the whole sample: χ = 37.615, P < 0.0001; in APOE e4 noncarriers: χ = 46.809, P < 0.0001). However, among APOE e4 carriers, prevalence of C carriers in CLOCK gene 3111T/C between patients and control subjects presents nonsignificant difference (χ = 0.812, P = 0.451). CONCLUSIONS: Among APOE e4 noncarriers, C carriers in CLOCK gene 3111T/C were associated with a high susceptibility of AD, but among APOE e4 carriers, the functional polymorphism of CLOCK gene 3111T/C was not associated with the susceptibility of AD.
OBJECTIVES: In the present study, it was examined whether polymorphism of circadian locomotor output cycle kaput (CLOCK) gene 3111T/C was associated with susceptibility of Alzheimer disease (AD). METHODS: This study was conducted using a case-control method. Genotypes of apolipoprotein E (APOE e4) and CLOCK gene 3111T/C were determined by restriction fragment length polymorphism method and TaqMan assay method, respectively. RESULTS: In this study, we gathered 296 unrelated ADpatients and 423 control subjects. Both in the whole sample and APOE e4 noncarriers, prevalence of C carriers in CLOCK gene 3111T/C in ADpatients was significantly higher than that in control subjects (in the whole sample: χ = 37.615, P < 0.0001; in APOE e4 noncarriers: χ = 46.809, P < 0.0001). However, among APOE e4 carriers, prevalence of C carriers in CLOCK gene 3111T/C between patients and control subjects presents nonsignificant difference (χ = 0.812, P = 0.451). CONCLUSIONS: Among APOE e4 noncarriers, C carriers in CLOCK gene 3111T/C were associated with a high susceptibility of AD, but among APOE e4 carriers, the functional polymorphism of CLOCK gene 3111T/C was not associated with the susceptibility of AD.
Authors: Eric A Mosser; Cindy N Chiu; T Katherine Tamai; Tsuyoshi Hirota; Suna Li; May Hui; Amy Wang; Chanpreet Singh; Andrew Giovanni; Steve A Kay; David A Prober Journal: Sci Rep Date: 2019-08-27 Impact factor: 4.379