| Literature DB >> 23911729 |
Shanshan Jiang1, Dan Zhang, Li Wang, Jiaowen Pan, Yang Liu, Xiangpei Kong, Yan Zhou, Dequan Li.
Abstract
Calcium-dependent protein kinases (CDPKs) play essential roles in calcium-mediated signal transductions in plant response to abiotic stress. Several members have been identified to be regulators for plants response to abscisic acid (ABA) signaling. Here, we isolated a subgroup I CDPK gene, ZmCPK4, from maize. Quantitative real time PCR (qRT-PCR) analysis revealed that the ZmCPK4 transcripts were induced by various stresses and signal molecules. Transient and stable expression of the ZmCPK4-GFP fusion proteins revealed ZmCPK4 localized to the membrane. Moreover, overexpression of ZmCPK4 in the transgenic Arabidopsis enhanced ABA sensitivity in seed germination, seedling growth and stomatal movement. The transgenic plants also enhanced drought stress tolerance. Taken together, the results suggest that ZmCPK4 might be involved in ABA-mediated regulation of stomatal closure in response to drought stress.Entities:
Keywords: ABA; Abscisic acid; CaMV; Calcium-dependent protein kinase gene; Drought stress; GFP; LA; MAPKs; MS; Maize; MeJA; Murashige-Skoog; ROS; WT; abscisic acid; cauliflower mosaic virus; green fluorescent protein; linolenic acid; methyl jasmonate; mitogen activated protein kinases; reactive oxygen species; wild-type
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Year: 2013 PMID： 23911729 DOI： 10.1016/j.plaphy.2013.07.004
Source DB: PubMed Journal: Plant Physiol Biochem ISSN： 0981-9428 Impact factor: 4.270