Literature DB >> 239077

Polymorphonuclear leukocyte phagocytosis: quantitation by a rapid radioactive method.

D S Miller, S Beck.   

Abstract

A rapid quantitative assay of phagocytosis has been developed. The method has been used to determine the phagocytic capability of human or dog polymorphonuclear leukocytes (PMN's) in a defined medium without added sera. The engulfment of 3H-labeled Staphylococcus albus is essentially complete in 30 minutes at 37 degrees C. Increase of phagocyte radioactivity is directly proportional to PMN per cubic centimeter over a wide range of cell concentrations. Maximal phagocytosis is observed at pH 7.5.

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Year:  1975        PMID： 239077

Source DB:  PubMed          Journal:  J Lab Clin Med        ISSN： 0022-2143


  5 in total

1.  Opsonic requirements for phagocytosis of Streptococcus pneumoniae types VI, XVIII, XXIII, and XXV.

Authors:  G S Giebink; J Verhoef; P K Peterson; P G Quie
Journal:  Infect Immun       Date:  1977-11       Impact factor: 3.441

2.  Opsonic requirements for staphylococcal phagocytosis. Heterogeneity among strains.

Authors:  J Verhoef; P Peterson; Y Kim; L D Sabath; P G Quie
Journal:  Immunology       Date:  1977-08       Impact factor: 7.397

Review 3.  Assessment of neutrophil function--II. Laboratory tests of neutrophil function.

Authors:  E N Wardle
Journal:  Postgrad Med J       Date:  1986-12       Impact factor: 2.401

4.  Extracellular and bacterial factors influencing staphylococcal phagocytosis and killing by human polymorphonuclear leukocytes.

Authors:  P K Peterson; J Verhoef; L D Sabath; P G Quie
Journal:  Infect Immun       Date:  1976-08       Impact factor: 3.441

5.  Estimation of phagocytic activity of polymorphonuclear leukocytes in chronic and aggressive periodontitis patients with nitroblue tetrazolium test.

Authors:  Jagadish Reddy Gooty; Andem Shashirekha; Vikram Reddy Guntakala; Rajababu Palaparthi
Journal:  J Indian Soc Periodontol       Date:  2019 Jul-Aug
  5 in total

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