| Literature DB >> 23906910 |
Melanie Krebs1, Karin Schumacher.
Abstract
Approaches for Ca(2+) measurement require data recording with high spatiotemporal resolution. This is achieved by combined usage of locally targeted genetically encoded calcium indicators (GECIs) and confocal laser scanning microscopy (CLSM). This protocol provides instructions for setting up a CLSM-based experiment to measure cytosolic and nuclear Ca(2+) dynamics in Arabidopsis lines expressing Yellow Cameleon Ca(2+) indicators.Entities:
Mesh：
Substances：
Year: 2013 PMID： 23906910 DOI： 10.1101/pdb.prot073031
Source DB: PubMed Journal: Cold Spring Harb Protoc ISSN： 1559-6095