Literature DB >> 2390450

31P NMR of mammalian cells encapsulated in alginate gels utilizing a new phosphate-free perfusion medium.

K S Narayan1, E A Moress, J C Chatham, P B Barker.   

Abstract

A simple technique permitting long term 31P magnetic resonance spectroscopy of cultured mammalian cells is described. Cells are encapsulated in calcium alginate gels and perfused with a new phosphate-fre medium designed for maintenance of high cell viability. Intracellular pH and beta-NTP/Pi ratios, studied in three cell types, remained stable over a 16-20 h NMR monitoring period. The techniques described may be useful for monitoring cell metabolism with a variety of cell types.

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Year:  1990        PMID： 2390450     DOI： 10.1002/nbm.1940030104

Source DB:  PubMed          Journal:  NMR Biomed        ISSN： 0952-3480            Impact factor:   4.044


  7 in total

1.  HGF/SF activates glycolysis and oxidative phosphorylation in DA3 murine mammary cancer cells.

Authors:  O Kaplan; M Firon; A Vivi; G Navon; I Tsarfaty
Journal:  Neoplasia       Date:  2000 Jul-Aug       Impact factor: 5.715

Review 2.  New advances in MR-compatible bioartificial liver.

Authors:  Rex E Jeffries; Jeffrey M Macdonald
Journal:  NMR Biomed       Date:  2011-02-20       Impact factor: 4.044

3.  Hyperpolarized (13)C spectroscopy and an NMR-compatible bioreactor system for the investigation of real-time cellular metabolism.

Authors:  Kayvan R Keshari; John Kurhanewicz; Rex E Jeffries; David M Wilson; Brian J Dewar; Mark Van Criekinge; Matthew Zierhut; Daniel B Vigneron; Jeffrey M Macdonald
Journal:  Magn Reson Med       Date:  2010-02       Impact factor: 4.668

4.  Towards the development of a bioartificial pancreas: immunoisolation and NMR monitoring of mouse insulinomas.

Authors:  A Sambanis; K K Papas; P C Flanders; R C Long; H Kang; I Constantinidis
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

Review 5.  Metabolism of breast cancer cells as revealed by non-invasive magnetic resonance spectroscopy studies.

Authors:  O Kaplan; J S Cohen
Journal:  Breast Cancer Res Treat       Date:  1994       Impact factor: 4.872

Review 6.  Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR.

Authors:  Enrico Luchinat; Matteo Cremonini; Lucia Banci
Journal:  Chem Rev       Date:  2022-01-21       Impact factor: 72.087

7.  Determination of Intra- and Extracellular Metabolic Adaptations of 3D Cell Cultures upon Challenges in Real-Time by NMR.

Authors:  Christian Urzì; Damian Hertig; Christoph Meyer; Sally Maddah; Jean-Marc Nuoffer; Peter Vermathen
Journal:  Int J Mol Sci       Date:  2022-06-12       Impact factor: 6.208
  7 in total

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