| Literature DB >> 2390214 |
M Baudys1, B Meloun, T Gan-Erdene, J Pohl, V Kostka.
Abstract
Bovine spleen cathepsin B contains 7 disulfide bridges. Cleavage of the enzyme with cyanogen bromide gives rise to a large and a small fragment. The former contains all disulfide bridges. Their arrangement was determined by analysis of amino-acid sequences and compositions of subfragments prepared by cleavage of the large cyanogen-bromide fragment with trypsin, chymotrypsin and the staphylococcal proteinase using specific methods for the detection of S-S-bonds. Disulfide bridges link together Cys14-Cys43, Cys26-Cys71, Cys62-Cys128, Cys63-Cys67, Cys100-Cys132, Cys108-Cys119 and Cys148-Cys252.Entities:
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Year: 1990 PMID： 2390214 DOI： 10.1515/bchm3.1990.371.1.485
Source DB: PubMed Journal: Biol Chem Hoppe Seyler ISSN： 0177-3593