John Penders1, Kerstin Gerhold2, Ellen E Stobberingh3, Carel Thijs4, Kurt Zimmermann5, Susanne Lau2, Eckard Hamelmann6. 1. Department of Medical Microbiology, School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands; Department of Epidemiology, School for Public Health and Primary Care, Maastricht University, Maastricht, The Netherlands. Electronic address: j.penders@maastrichtuniversity.nl. 2. Department of Pediatric Pneumology and Immunology, Charité - Universitätsmedizin Berlin, Berlin, Germany. 3. Department of Medical Microbiology, School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands. 4. Department of Epidemiology, School for Public Health and Primary Care, Maastricht University, Maastricht, The Netherlands. 5. SymbioPharm, Herborn, Germany. 6. University Children's Hospital, Ruhr-Universität Bochum, Bochum, Germany.
Abstract
BACKGROUND: Perturbations in the intestinal microbiota may disrupt mechanisms involved in the development of immunologic tolerance. The present study aimed to examine the establishment of the infant microbiota and its association to the development of atopic dermatitis (AD). METHODS: Within a randomized, placebo-controlled trial on the prevention of AD by oral supplementation of a bacterial lysate between week 5 and the end of month 7, feces was collected at the ages of 5 weeks (n = 571), 13 weeks (n = 332), and 31 weeks (n = 499) and subjected to quantitative PCRs to detect bifidobacteria, bacteroides, lactobacilli, Escherichia coli, Clostridium difficile, and Clostridium cluster I. RESULTS: Birth mode, breast-feeding but also birth order had a strong effect on the microbiota composition. With increasing number of older siblings the colonization rates at age 5 weeks of lactobacilli (P < .001) and bacteroides (P = .02) increased, whereas rates of clostridia decreased (P < .001). Colonization with clostridia, at the age of 5 and 13 weeks was also associated with an increased risk of developing AD in the subsequent 6 months of life (odds ratioadjusted = 2.35; 95% CI, 1.36-3.94 and 2.51; 1.30-4.86, respectively). Mediation analyses demonstrated that there was a statistically significant indirect effect via Clostridium cluster I colonization for both birth mode and birth order in association to AD. CONCLUSION: The results of this study are supportive for a role of the microbiota in the development of AD. Moreover, the "beneficial" influence of older siblings on the microbiota composition suggests that this microbiota may be one of the biological mechanisms underlying the sibling effect.
RCT Entities:
BACKGROUND: Perturbations in the intestinal microbiota may disrupt mechanisms involved in the development of immunologic tolerance. The present study aimed to examine the establishment of the infant microbiota and its association to the development of atopic dermatitis (AD). METHODS: Within a randomized, placebo-controlled trial on the prevention of AD by oral supplementation of a bacterial lysate between week 5 and the end of month 7, feces was collected at the ages of 5 weeks (n = 571), 13 weeks (n = 332), and 31 weeks (n = 499) and subjected to quantitative PCRs to detect bifidobacteria, bacteroides, lactobacilli, Escherichia coli, Clostridium difficile, and Clostridium cluster I. RESULTS: Birth mode, breast-feeding but also birth order had a strong effect on the microbiota composition. With increasing number of older siblings the colonization rates at age 5 weeks of lactobacilli (P < .001) and bacteroides (P = .02) increased, whereas rates of clostridia decreased (P < .001). Colonization with clostridia, at the age of 5 and 13 weeks was also associated with an increased risk of developing AD in the subsequent 6 months of life (odds ratioadjusted = 2.35; 95% CI, 1.36-3.94 and 2.51; 1.30-4.86, respectively). Mediation analyses demonstrated that there was a statistically significant indirect effect via Clostridium cluster I colonization for both birth mode and birth order in association to AD. CONCLUSION: The results of this study are supportive for a role of the microbiota in the development of AD. Moreover, the "beneficial" influence of older siblings on the microbiota composition suggests that this microbiota may be one of the biological mechanisms underlying the sibling effect.
Authors: Kohei Hasegawa; Rachel W Linnemann; Jonathan M Mansbach; Nadim J Ajami; Janice A Espinola; Lauren G Fiechtner; Joseph F Petrosino; Carlos A Camargo Journal: Pediatr Int Date: 2016-12-22 Impact factor: 1.524
Authors: Joanne E Sordillo; Yanjiao Zhou; Michael J McGeachie; John Ziniti; Nancy Lange; Nancy Laranjo; Jessica R Savage; Vincent Carey; George O'Connor; Megan Sandel; Robert Strunk; Leonard Bacharier; Robert Zeiger; Scott T Weiss; George Weinstock; Diane R Gold; Augusto A Litonjua Journal: J Allergy Clin Immunol Date: 2016-10-13 Impact factor: 10.793