| Literature DB >> 23896375 |
Monika Praschberger1, Marcela Hermann, Christian Laggner, Leopold Jirovetz, Markus Exner, Stylianos Kapiotis, Bernhard M K Gmeiner, Hilde Laggner.
Abstract
Hydrogen sulfide (H2S) has been identified as the third gasotransmitter. Beside its role as signaling molecule in the cardiovascular and nervous system the antioxidant and cyto-protective properties of H2S have gained much attention. In the present study we show that cyanate, an uremic toxin which is found in abundant concentration in sera of patients suffering from chronic kidney disease (CKD), can abrogate the antioxidant and cytoprotective activity of H2S via S-carbamoylation reaction, a reaction that previously has only been shown to have a physiological effect on cysteine groups, but not on H2S. Carbamoylation strongly inhibited the free radical scavenging (ABTS(+·) and alkylperoxyl ROO(·)) properties of H2S. The extent of intracellular ROS formation induced by ROO(·) was diminished by H2S whereas carbamoylation counteracted the protective effect. Reagent HOCl was rapidly inactivated by H2S in contrast to the carbamoylated compound. Protein modification by HOCl was inhibited by H2S but carbamoylation significantly reduced the effect. Thus, S-carbamoylation of low molecular weight thiols by abrogating their antioxidant potential may contribute to the higher oxidative stress observed in CKD.Entities:
Keywords: (3,3′,5,5′-tetramethylbenzidine); 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt; 2,2′-azobis(2-amidinopropane) hydrochloride; 2′,7′-dichlorofluorescein; AAPH; ABTS; Antioxidant; Chronic kidney disease; Cyanate; DCF; Hydrogen sulfide; Isocyanic acid; S-Carbamoylation; TMB
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Year: 2013 PMID： 23896375 DOI： 10.1016/j.biochi.2013.07.018
Source DB: PubMed Journal: Biochimie ISSN： 0300-9084 Impact factor: 4.079