OBJECTIVE: To evaluate the prevalence of human papillomavirus (HPV) sperm infection and its correlation with sperm parameters in patients who attended a fertility clinic. DESIGN: Cross-sectional clinical study. SETTING: University-affiliated reproductive medicine clinic. PATIENT(S): A total of 308 male partners of couples undergoing in vitro fertilization techniques. INTERVENTION(S): Specimens of semen were collected from all patients. MAIN OUTCOME MEASURE(S): Sperm parameters were evaluated according to the World Health Organization manual. The presence of HPV DNA was researched by the combined use of two HPV assays and a highly sensitive nested polymerase chain reaction assay followed by HPV genotyping. To examine whether HPV was associated with the sperm, in situ hybridization (ISH) analysis was performed. RESULT(S): Results of HPV investigation were compared with sperm parameters and ISH analysis. Twenty-four out of 308 semen samples (7.8%) were HPV DNA positive, but HPV infection did not seem to affect semen quality. Moreover, ISH revealed a clear HPV localization at the equatorial region of sperm head in infected samples. CONCLUSION(S): Oncogenic HPV genotypes were detected on spermatozoa from asymptomatic subjects, but a role of the infection in male infertility was not demonstrated.
OBJECTIVE: To evaluate the prevalence of human papillomavirus (HPV) sperm infection and its correlation with sperm parameters in patients who attended a fertility clinic. DESIGN: Cross-sectional clinical study. SETTING: University-affiliated reproductive medicine clinic. PATIENT(S): A total of 308 male partners of couples undergoing in vitro fertilization techniques. INTERVENTION(S): Specimens of semen were collected from all patients. MAIN OUTCOME MEASURE(S): Sperm parameters were evaluated according to the World Health Organization manual. The presence of HPV DNA was researched by the combined use of two HPV assays and a highly sensitive nested polymerase chain reaction assay followed by HPV genotyping. To examine whether HPV was associated with the sperm, in situ hybridization (ISH) analysis was performed. RESULT(S): Results of HPV investigation were compared with sperm parameters and ISH analysis. Twenty-four out of 308 semen samples (7.8%) were HPV DNA positive, but HPV infection did not seem to affect semen quality. Moreover, ISH revealed a clear HPV localization at the equatorial region of sperm head in infected samples. CONCLUSION(S): Oncogenic HPV genotypes were detected on spermatozoa from asymptomatic subjects, but a role of the infection in male infertility was not demonstrated.
Authors: Edilson Damke; Fábio A Kurscheidt; Valério A Balani; Karen I Takeda; Mary M T Irie; Fabrícia Gimenes; Marcia E L Consolaro Journal: Biomed Res Int Date: 2017-08-01 Impact factor: 3.411
Authors: Nigel Pereira; Katherine M Kucharczyk; Jaclyn L Estes; Rachel S Gerber; Jovana P Lekovich; Rony T Elias; Steven D Spandorfer Journal: J Pathog Date: 2015-11-01
Authors: Barbara Golob; Mario Poljak; Ivan Verdenik; Mojca Kolbezen Simoniti; Eda Vrtačnik Bokal; Branko Zorn Journal: Biomed Res Int Date: 2014-04-06 Impact factor: 3.411
Authors: Elena Lopez-Diez; Sonia Perez; Manuel Carballo; Amparo Iñarrea; Angel de la Orden; Maximo Castro; Moises Rodríguez; Sheila Almuster; Ruben Montero; Miguel Perez; Jorge Sanchez; Antonio Ojea Journal: PLoS One Date: 2017-09-12 Impact factor: 3.240