Literature DB >> 2388829

The degradation of T7 DNA in converging flow.

B H Zimm1, H R Reese.   

Abstract

The flow-induced degradation of T7 DNA (Molecular Size = 40 Kbp) was studied in a flow device that generates converging flow rather than simple shear flow. We discovered that the sizes of the degradation products were very broadly distributed, covering the range from 10 Kbp to 36 Kbp. An explanation for the broadness of the distribution is given based on a computer simulation of the experiment. The significance of converging flow to the routine handling of large DNA is emphasized.

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Year:  1990        PMID： 2388829      PMCID： PMC331266          DOI： 10.1093/nar/18.15.4469

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  3 in total

1.  THE EFFECT OF HYDRODYNAMIC SHEAR ON THE DEOXYRIBONUCLEIC ACID FROM T(2) AND T(4) BACTERIOPHAGES.

Authors:  P F Davison
Journal:  Proc Natl Acad Sci U S A       Date:  1959-11       Impact factor: 11.205

2.  Hydrodynamic shear breakage of DNA.

Authors:  R D Bowman; N Davidson
Journal:  Biopolymers       Date:  1972       Impact factor: 2.505

3.  Shear degradation of DNA.

Authors:  R E Adam; B H Zimm
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971
  3 in total
  3 in total

1.  The conformation of DNA packaged in bacteriophage G.

Authors:  M Sun; P Serwer
Journal:  Biophys J       Date:  1997-02       Impact factor: 4.033

2.  Video light microscopy of 670-kb DNA in a hanging drop: shape of the envelope of DNA.

Authors:  P Serwer; A Estrada; R A Harris
Journal:  Biophys J       Date:  1995-12       Impact factor: 4.033

3.  Effects of temperature on excluded volume-promoted cyclization and concatemerization of cohesive-ended DNA longer than 0.04 Mb.

Authors:  D Louie; P Serwer
Journal:  Nucleic Acids Res       Date:  1991-06-11       Impact factor: 16.971
  3 in total

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