L Max Almond1, Jo Hutchings2, Gavin Lloyd2, Hugh Barr1, Neil Shepherd3, John Day4, Oliver Stevens4, Scott Sanders5, Martin Wadley6, Nick Stone7, Catherine Kendall2. 1. Biophotonics Research Unit, Gloucestershire Hospitals NHS Foundation Trust, Gloucester, United Kingdom; Department of Esophagogastric Surgery, Gloucestershire Hospitals NHS Foundation Trust, Gloucester, United Kingdom. 2. Biophotonics Research Unit, Gloucestershire Hospitals NHS Foundation Trust, Gloucester, United Kingdom. 3. Department of Histopathology, Gloucestershire Hospitals NHS Foundation Trust, Gloucester, United Kingdom. 4. Interface Analysis Centre, University of Bristol, Bristol, United Kingdom. 5. Department of Histopathology, Warwick Hospital, Warwick, United Kingdom. 6. Department of Esophagogastric Surgery, Gloucestershire Hospitals NHS Foundation Trust, Gloucester, United Kingdom; Department of Upper Gastrointestinal Surgery, Worcestershire Hospitals NHS Trust, Worcester, United Kingdom. 7. Department of Physics, Exeter University, Exeter, United Kingdom.
Abstract
BACKGROUND: Early detection and targeted endoscopic resection of Barrett's esophagus-associated high-grade dysplasia (HGD) can prevent progression to invasive esophageal malignancy. Raman spectroscopy, a highly sophisticated analytical technique, has been translated into an endoscopic tool to facilitate rapid, objective diagnosis of dysplasia in the esophagus. OBJECTIVE: To evaluate the ability of endoscopic Raman spectroscopy (ERS) to objectively detect esophageal HGD and adenocarcinoma. DESIGN: A total of 798 one-second spectra were measured from 673 ex vivo esophageal tissue samples, collected from patients with Barrett's esophagus by using a novel endoscopic Raman probe. Spectra were correlated with consensus histopathology. Multivariate analysis was used to evaluate the classification accuracy of ERS ex vivo. SETTING: Probe measurements were conducted in the laboratory. Tissue specimens were collected from the operating theatre and endoscopy unit. PATIENTS: Tissue from 62 patients was included in the study. INTERVENTIONS: Endoscopic biopsy/resection or esophagectomy was performed where indicated clinically. MAIN OUTCOME MEASUREMENT: Diagnostic performance of ERS for detection of HGD and esophageal adenocarcinoma. RESULTS: ERS demonstrated a sensitivity of 86% and a specificity of 88% for detecting HGD and adenocarcinoma. The ability to grade dysplasia and differentiate intestinal metaplasia from nonintestinal metaplasia columnar-lined esophagus was also demonstrated. Diagnostic classification was based on objective measurement of the biochemical profile of different tissue types. The potential for combination ERS and narrow-band imaging was also demonstrated. LIMITATIONS: Measurements were taken from ex vivo tissue. CONCLUSION: ERS enables rapid, accurate, objective diagnosis of superficial esophageal disease (metaplasia, dysplasia, intramucosal cancer) in clinically applicable time scales. Crown
BACKGROUND: Early detection and targeted endoscopic resection of Barrett's esophagus-associated high-grade dysplasia (HGD) can prevent progression to invasive esophageal malignancy. Raman spectroscopy, a highly sophisticated analytical technique, has been translated into an endoscopic tool to facilitate rapid, objective diagnosis of dysplasia in the esophagus. OBJECTIVE: To evaluate the ability of endoscopic Raman spectroscopy (ERS) to objectively detect esophageal HGD and adenocarcinoma. DESIGN: A total of 798 one-second spectra were measured from 673 ex vivo esophageal tissue samples, collected from patients with Barrett's esophagus by using a novel endoscopic Raman probe. Spectra were correlated with consensus histopathology. Multivariate analysis was used to evaluate the classification accuracy of ERS ex vivo. SETTING: Probe measurements were conducted in the laboratory. Tissue specimens were collected from the operating theatre and endoscopy unit. PATIENTS: Tissue from 62 patients was included in the study. INTERVENTIONS: Endoscopic biopsy/resection or esophagectomy was performed where indicated clinically. MAIN OUTCOME MEASUREMENT: Diagnostic performance of ERS for detection of HGD and esophageal adenocarcinoma. RESULTS: ERS demonstrated a sensitivity of 86% and a specificity of 88% for detecting HGD and adenocarcinoma. The ability to grade dysplasia and differentiate intestinal metaplasia from nonintestinal metaplasia columnar-lined esophagus was also demonstrated. Diagnostic classification was based on objective measurement of the biochemical profile of different tissue types. The potential for combination ERS and narrow-band imaging was also demonstrated. LIMITATIONS: Measurements were taken from ex vivo tissue. CONCLUSION: ERS enables rapid, accurate, objective diagnosis of superficial esophageal disease (metaplasia, dysplasia, intramucosal cancer) in clinically applicable time scales. Crown
Authors: Jordan A Sweer; Mason T Chen; Kevan J Salimian; Richard J Battafarano; Nicholas J Durr Journal: J Biophotonics Date: 2019-06-14 Impact factor: 3.207
Authors: Saleh Ramezani; Arianna Parkhideh; Pratip K Bhattacharya; Mary C Farach-Carson; Daniel A Harrington Journal: Front Oncol Date: 2021-07-05 Impact factor: 6.244