Literature DB >> 23872007

Development of buffers for fast semidry transfer of proteins.

Dušan Garić1, Laure Humbert, Nadège Fils-Aimé, Juliana Korah, Yasaman Zarfabian, Jean-Jacques Lebrun, Suhad Ali.   

Abstract

Western blot is an extensively used method for protein detection in cell biology. To optimize this procedure, here we examined a panel of buffers for their ability to efficiently transfer proteins from SDS-polyacrylamide gels onto nitrocellulose membranes in a short 12-min period, designated here as fast semidry transfer. Our results show for the first time that HEPES- and HEPPS/EPPS-based buffers represent the most efficient buffers for fast semidry transfer.
Copyright © 2013 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  HEPES; HEPPS; Protein transfer; Western blot

Mesh：

Substances：

Year:  2013        PMID： 23872007     DOI： 10.1016/j.ab.2013.07.009

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  5 in total

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  5 in total

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