OBJECTIVES: To evaluate the prevalence and circulation of rotavirus genotypes before and after the introduction of oral vaccine against human rotavirus, and to check for a possible change in the age of occurence of the infection by RV-A. METHODS: This was a cross-sectional study conducted between 2002-2011, in the city of Juiz de Fora, state of Minas Gerais, Brazil. A total of 1,144 diarrheal stool specimens were obtained from nonhospitalized children aged between 0 and 5 years, and analyzed by polyacrylamide gel electrophoresis and reverse-transcription polymerase chain reaction for genotype characterization. Data on prevalence and age distribution of rotavirus cases were analyzed through the chi-squared test (p < 0.05), using SPSS, release 13.0. RESULTS: Rotavirus infection was detected in 9.35% (107/1,144) samples, with prevalence rates ranging from 11.12% (90/809) in the pre-vaccine to 5.07% (17/335) in the post-vaccine period (p = 0.001). Among the samples tested, the most frequently detected genotypes were G1P[6] (6/33 = 18.2%) in the period between 2002 and 2005 and G2P[4] in 2006 (11/33 = 33.3%) and in the period between 2007 and 2011 (5/33 = 15.2%). There was also a significant reduction in the number of cases of rotavirus disease in children aged between 0 and 36 months after the vaccine introduction. CONCLUSIONS: The study evidenced a significant decrease in the prevalence of rotavirus, mainly in children aged between 0 and 36 months in the 2007-2011 period, as well as a reduction in G1 genotype circulation.
OBJECTIVES: To evaluate the prevalence and circulation of rotavirus genotypes before and after the introduction of oral vaccine against human rotavirus, and to check for a possible change in the age of occurence of the infection by RV-A. METHODS: This was a cross-sectional study conducted between 2002-2011, in the city of Juiz de Fora, state of Minas Gerais, Brazil. A total of 1,144 diarrheal stool specimens were obtained from nonhospitalized children aged between 0 and 5 years, and analyzed by polyacrylamide gel electrophoresis and reverse-transcription polymerase chain reaction for genotype characterization. Data on prevalence and age distribution of rotavirus cases were analyzed through the chi-squared test (p < 0.05), using SPSS, release 13.0. RESULTS:Rotavirus infection was detected in 9.35% (107/1,144) samples, with prevalence rates ranging from 11.12% (90/809) in the pre-vaccine to 5.07% (17/335) in the post-vaccine period (p = 0.001). Among the samples tested, the most frequently detected genotypes were G1P[6] (6/33 = 18.2%) in the period between 2002 and 2005 and G2P[4] in 2006 (11/33 = 33.3%) and in the period between 2007 and 2011 (5/33 = 15.2%). There was also a significant reduction in the number of cases of rotavirus disease in children aged between 0 and 36 months after the vaccine introduction. CONCLUSIONS: The study evidenced a significant decrease in the prevalence of rotavirus, mainly in children aged between 0 and 36 months in the 2007-2011 period, as well as a reduction in G1 genotype circulation.
Authors: Andrêssa Silvino Ferreira Assis; Lucas Taffarel Cruz; Aline Siqueira Ferreira; Martha Eunice Bessa; Miriam Aparecida de Oliveira Pinto; Carmen Baur Vieira; Marcelo Henrique Otenio; Marize Pereira Miagostovich; Maria Luzia da Rosa E Silva Journal: Environ Sci Pollut Res Int Date: 2014-12-04 Impact factor: 4.223
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Authors: Jie Liu; Kate Lurain; Shihab U Sobuz; Sharmin Begum; Happiness Kumburu; Jean Gratz; Gibson Kibiki; Denise Toney; Rashi Gautam; Michael D Bowen; William A Petri; Rashidul Haque; Eric R Houpt Journal: J Virol Methods Date: 2014-12-17 Impact factor: 2.014
Authors: Abdul Momin Kazi; Gohar Javed Warraich; Shahida Qureshi; Huma Qureshi; Muhammad Mubashir Ahmad Khan; Anita Kaniz Mehdi Zaidi Journal: PLoS One Date: 2014-10-08 Impact factor: 3.240
Authors: Shabihah Shahrudin; Cheng Chen; Shannon C David; Eve V Singleton; Justin Davies; Carl D Kirkwood; Timothy R Hirst; Michael Beard; Mohammed Alsharifi Journal: PLoS One Date: 2018-06-07 Impact factor: 3.240
Authors: Thaís Aparecida Vieira Reis; Andrêssa Silvino Ferreira Assis; Daniel Almeida do Valle; Vívian Honorato Barletta; Iná Pires de Carvalho; Tatiana Lundgren Rose; Silvana Augusta Rodrigues Portes; José Paulo Gagliardi Leite; Maria Luzia da Rosa e Silva Journal: Braz J Microbiol Date: 2016-01-27 Impact factor: 2.476