| Literature DB >> 23870948 |
Katarína Kozics1, Veronika Klusová, Annamária Srančíková, Pavol Mučaji, Darina Slameňová, Lubica Hunáková, Barbara Kusznierewicz, Eva Horváthová.
Abstract
Salvia officinalis (SO) and Thymus vulgaris (TV) are medicinal plants well known for their curative powers. However, the molecular mechanisms responsible for these abilities of sage and thyme have not been fully understood yet. In this study we investigated the composition and the quantitative estimation of plant extracts, the protective effects of plant extracts against hydrogen peroxide- and 2,3-dimethoxy-1,4-naphthoquinone-induced DNA damage, and levels of enzymatic and non-enzymatic antioxidants (superoxide dismutase, glutathione peroxidase, glutathione) in human HepG2 cells. To measure antioxidative activity of plant extracts we used three assays: 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The results showed that the oxidant-induced DNA lesions were significantly reduced in cells pre-treated with the plant extracts studied. The observed DNA-protective activity could be explained by both elevation of GPx activity in cells pre-treated with SO and TV and antioxidant activity of SO and TV.Entities:
Keywords: Antioxidant activity; Antioxidant enzymes; Comet assay; Human hepatoma HepG2 cells; Salvia officinalis extract; Thymus vulgaris extract
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Year: 2013 PMID： 23870948 DOI： 10.1016/j.foodchem.2013.04.089
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514