OBJECTIVE: To explore the roles of osteopontin and β3 integrin in successful implantation. STUDY DESIGN: In this study, an early pregnant mouse model was established by peritoneal injection of pregnant mare serum gonadotropin and human chorionic gonadotropin (PMSG+hCG). The expression of osteopontin (OPN) and β3 integrin on the endometrium was measured by immunohistochemistry, RT-PCR, and western blot. The function of OPN and β3 integrin in implantation was investigated by intrauterine injection of OPN and β3 integrin antibody. RESULTS: We found that PMSG+hCG injection significantly increased the number of blastocysts during implantation as well as the concentration of estradiol and progesterone in serum and endometrium tissues. OPN and β3 integrin were co-expressed in luminal epithelium and their levels dynamically changed from day 4 to day 8 of pregnancy with peak expression on day 5. The percentages of OPN and β3 integrin positive cells in the luminal epithelium were significantly higher in PMSG+hCG-stimulated mice on day 5 than in control mice. Functional blockade of OPN and β3 integrin significantly inhibited implantation. CONCLUSIONS: This study suggests that co-expression of OPN and β3 integrin is a biological marker for good endometrial receptivity and that both proteins play a crucial role in blastocyst implantation.
OBJECTIVE: To explore the roles of osteopontin and β3 integrin in successful implantation. STUDY DESIGN: In this study, an early pregnant mouse model was established by peritoneal injection of pregnant mare serum gonadotropin and human chorionic gonadotropin (PMSG+hCG). The expression of osteopontin (OPN) and β3 integrin on the endometrium was measured by immunohistochemistry, RT-PCR, and western blot. The function of OPN and β3 integrin in implantation was investigated by intrauterine injection of OPN and β3 integrin antibody. RESULTS: We found that PMSG+hCG injection significantly increased the number of blastocysts during implantation as well as the concentration of estradiol and progesterone in serum and endometrium tissues. OPN and β3 integrin were co-expressed in luminal epithelium and their levels dynamically changed from day 4 to day 8 of pregnancy with peak expression on day 5. The percentages of OPN and β3 integrin positive cells in the luminal epithelium were significantly higher in PMSG+hCG-stimulated mice on day 5 than in control mice. Functional blockade of OPN and β3 integrin significantly inhibited implantation. CONCLUSIONS: This study suggests that co-expression of OPN and β3 integrin is a biological marker for good endometrial receptivity and that both proteins play a crucial role in blastocyst implantation.
Authors: Brigitta Dombai; István Ivancsó; András Bikov; Dóra Oroszi; Anikó Bohács; Veronika Müller; János Rigó; Barna Vásárhelyi; György Losonczy; Lilla Tamási Journal: Can Respir J Date: 2017-10-23 Impact factor: 2.409
Authors: Stéphane C Berneau; Peter T Ruane; Daniel R Brison; Susan J Kimber; Melissa Westwood; John D Aplin Journal: Cells Date: 2019-05-09 Impact factor: 6.600
Authors: Silvia León; Daniela Fernandois; Alexandra Sull; Judith Sull; Michele Calder; Kanako Hayashi; Moshmi Bhattacharya; Stephen Power; George A Vilos; Angelos G Vilos; Manuel Tena-Sempere; Andy V Babwah Journal: Sci Rep Date: 2016-07-01 Impact factor: 4.379