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Literature DB >> 2386434

Vaccinia virus expression and sequence of an avian influenza nucleoprotein gene: potential use in diagnosis.

V R Harley¹, P J Hudson, B E Coupar, P W Selleck, H Westbury, D B Boyle.

Abstract

The nucleoprotein (NP) gene from avian influenza strain A/Shearwater/Aust/1/72 (H6N5) was cloned, sequenced, and expressed in vaccinia virus for the production of potent sera in immunised rabbits. The NP gene is 1565 bp and shares greater than 95% amino acid sequence identity with other NPs of the avian subtype. The recombinant NP expressed by vaccinia virus comigrated with endogenous A/Shearwater/Aust/1/72 NP by Western blot analysis. Polyclonal rabbit sera raised against recombinant NP was evaluated in an antigen capture ELISA system as a potential diagnostic tool for the detection of avian influenza. All type A strains, comprising several HA and NA subtypes, but not type B nor other avian viruses, were detected.

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Year: 1990 PMID： 2386434 DOI： 10.1007/bf01318362

Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574

20 in total

1. The epitopes of influenza nucleoprotein recognized by cytotoxic T lymphocytes can be defined with short synthetic peptides.

Authors: A R Townsend; J Rothbard; F M Gotch; G Bahadur; D Wraith; A J McMichael
Journal: Cell Date: 1986-03-28 Impact factor: 41.582

2. Phylogenetic analysis of nucleoproteins suggests that human influenza A viruses emerged from a 19th-century avian ancestor.

Authors: M Gammelin; A Altmüller; U Reinhardt; J Mandler; V R Harley; P J Hudson; W M Fitch; C Scholtissek
Journal: Mol Biol Evol Date: 1990-03 Impact factor: 16.240

3. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.

Authors: E Y Chen; P H Seeburg
Journal: DNA Date: 1985-04

4. Synthesis and cellular location of the ten influenza polypeptides individually expressed by recombinant vaccinia viruses.

Authors: G L Smith; J Z Levin; P Palese; B Moss
Journal: Virology Date: 1987-10 Impact factor: 3.616

Vaccinia virus expression and sequence of an avian influenza nucleoprotein gene: potential use in diagnosis.

1. The epitopes of influenza nucleoprotein recognized by cytotoxic T lymphocytes can be defined with short synthetic peptides.

2. Phylogenetic analysis of nucleoproteins suggests that human influenza A viruses emerged from a 19th-century avian ancestor.

3. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.

4. Synthesis and cellular location of the ten influenza polypeptides individually expressed by recombinant vaccinia viruses.

5. Construction of recombinant fowlpox viruses as vectors for poultry vaccines.

6. General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes.

7. Enzyme-linked immunosorbent assay of core antigens for clinical diagnosis of influenza.

8. Antigenic variation of influenza A virus nucleoprotein detected with monoclonal antibodies.

9. Expression of influenza A virus internal antigens on the surface of infected P815 cells.

10. Multiple-cloning-site plasmids for the rapid construction of recombinant poxviruses.

1. Characterisation of an avian influenza virus nucleoprotein expressed in E. coli and in insect cells.

2. Nek4 regulates entry into replicative senescence and the response to DNA damage in human fibroblasts.

3. Detection of NP, N3 and N7 antibodies to avian influenza virus by indirect ELISA using yeast-expressed antigens.