Literature DB >> 23863571

UV-fixed-thick-blotch preparation improves sensitivity of auramine staining.

M A Cejudo-García1, M J Munoz-Davila, M J Martinez-Lirola.   

Abstract

We describe here a very simple modification of the auramine staining procedure based on preparation of a UV-fixed thick blotch which allowed us to reach an overall sensitivity of 0.82 (592 acid-fast bacillus [AFB]-positive specimens/722 initial respiratory specimens with positive mycobacterial culture) and sensitivities of 0.93 (526 AFB-positive specimens/564 culture-positive specimens) for Mycobacterium tuberculosis complex and 0.42 (66 AFB-positive specimens/158 culture-positive specimens) for nontuberculous mycobacteria.

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Year:  2013        PMID： 23863571      PMCID： PMC3811656          DOI： 10.1128/JCM.00974-13

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  6 in total

Review 1.  An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases.

Authors:  David E Griffith; Timothy Aksamit; Barbara A Brown-Elliott; Antonino Catanzaro; Charles Daley; Fred Gordin; Steven M Holland; Robert Horsburgh; Gwen Huitt; Michael F Iademarco; Michael Iseman; Kenneth Olivier; Stephen Ruoss; C Fordham von Reyn; Richard J Wallace; Kevin Winthrop
Journal:  Am J Respir Crit Care Med       Date:  2007-02-15       Impact factor: 21.405

Review 2.  Sputum processing methods to improve the sensitivity of smear microscopy for tuberculosis: a systematic review.

Authors:  Karen R Steingart; Vivienne Ng; Megan Henry; Philip C Hopewell; Andrew Ramsay; Jane Cunningham; Richard Urbanczik; Mark D Perkins; Mohamed Abdel Aziz; Madhukar Pai
Journal:  Lancet Infect Dis       Date:  2006-10       Impact factor: 25.071

3.  Present position of microscopy and of culture in diagnostic mycobacteriology.

Authors:  R Urbanczik
Journal:  Zentralbl Bakteriol Mikrobiol Hyg A       Date:  1985-08

4.  Diagnosis of tuberculosis and drug resistance: what can new tools bring us?

Authors:  F Drobniewski; V Nikolayevskyy; Y Balabanova; D Bang; D Papaventsis
Journal:  Int J Tuberc Lung Dis       Date:  2012-07       Impact factor: 2.373

5.  Transmission of Mycobacterium tuberculosis from patients smear-negative for acid-fast bacilli.

Authors:  M A Behr; S A Warren; H Salamon; P C Hopewell; A Ponce de Leon; C L Daley; P M Small
Journal:  Lancet       Date:  1999-02-06       Impact factor: 79.321

6.  Use of UV ParaLens adapter for detection of acid-fast organisms.

Authors:  K V Patterson; C L McDonald; B F Miller; K C Chapin
Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948
  6 in total
  2 in total

1.  Urgent Implementation in a Hospital Setting of a Strategy To Rule Out Secondary Cases Caused by Imported Extensively Drug-Resistant Mycobacterium tuberculosis Strains at Diagnosis.

Authors:  Laura Pérez-Lago; Miguel Martínez-Lirola; Sergio García; Marta Herranz; Igor Mokrousov; Iñaki Comas; Llúcia Martínez-Priego; Emilio Bouza; Darío García-de-Viedma
Journal:  J Clin Microbiol       Date:  2016-09-28       Impact factor: 5.948

2.  Ultraviolet light enhances the bovine serum albumin fixation for acid fast bacilli stain.

Authors:  Jun-Ren Sun; Yun-Hsiang Cheng; Pei-Yin Lai; Shih-Yi Lee; Yu-Ching Chou; Yung-Chieh Fu; Chen-Cheng Wu; Tzong-Shi Chiueh
Journal:  PLoS One       Date:  2014-02-21       Impact factor: 3.240
  2 in total

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