BACKGROUND: Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). METHODS: Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n = 28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. RESULTS: pDC (ie, CD303(+)/CD1c(-) PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-α (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). CONCLUSION: Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.
BACKGROUND: Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). METHODS: Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n = 28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. RESULTS: pDC (ie, CD303(+)/CD1c(-) PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-α (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). CONCLUSION: Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.
Authors: Meghan S Vermillion; Andrew Nelson; Landon Vom Steeg; Jeffery Loube; Wayne Mitzner; Sabra L Klein Journal: Am J Physiol Lung Cell Mol Physiol Date: 2018-05-31 Impact factor: 5.464
Authors: Alexander W Kay; Julia Fukuyama; Natali Aziz; Cornelia L Dekker; Sally Mackey; Gary E Swan; Mark M Davis; Susan Holmes; Catherine A Blish Journal: Proc Natl Acad Sci U S A Date: 2014-09-22 Impact factor: 11.205
Authors: Seunghee Hong; Romain Banchereau; Bat-Sheva L Maslow; Jacques Banchereau; Jane E Salmon; Virginia Pascual; Marta M Guerra; Jacob Cardenas; Jeanine Baisch; D Ware Branch; T Flint Porter; Allen Sawitzke; Carl A Laskin; Jill P Buyon; Joan Merrill; Lisa R Sammaritano; Michelle Petri; Elizabeth Gatewood; Alma-Martina Cepika; Marina Ohouo; Gerlinde Obermoser; Esperanza Anguiano; Tae Whan Kim; John Nulsen; Djamel Nehar-Belaid; Derek Blankenship; Jacob Turner Journal: J Exp Med Date: 2019-04-08 Impact factor: 14.307
Authors: Dominika Swieboda; Elizabeth Q Littauer; Jacob T Beaver; Lisa K Mills; Katherine M Bricker; E Stein Esser; Olivia Q Antao; Dahnide T Williams; Ioanna Skountzou Journal: Front Immunol Date: 2020-08-14 Impact factor: 7.561