BACKGROUND: We sought to determine the quantitative expression of human leukocyte antigen-DR (HLA-DR) on monocytes in patients with acute intestinal bacterial infections and inflammatory bowel disease (IBD). METHODS: The quantitative expression of HLA-DR on monocytes was determined by fluorescence-activated cell sorting analysis in patients with IBD, patients with acute intestinal bacterial infections (bact.), and healthy subjects (contr.). RESULTS: The quantitative expression of HLA-DR in patients with bact. (n = 20; 90,000 molecules per monocyte; confidence interval [CI], 79,000-102,000) was significantly higher than that in patients with ulcerative colitis (n = 40, 30,000; CI, 30,000-38,000; P < 0.0001), Crohn disease (n = 80, 31,000; CI, 32,000-39,000; P < 0.0001), or in contr. (n = 28, 39,000; CI, 36,000-46,000; P < 0.0001). In patients with ulcerative colitis and Crohn disease, HLA-DR expression was significantly decreased, as compared with contr. (P < 0.05 and P < 0.01, respectively). With a cutoff point of 50,000, HLA-DR showed a sensitivity of 95% and a specificity of 92% in discriminating between bact. and active IBD. CONCLUSION: The quantitative measurement of HLA-DR expression could serve as a valuable tool to discriminate between bact. and active IBD.
BACKGROUND: We sought to determine the quantitative expression of human leukocyte antigen-DR (HLA-DR) on monocytes in patients with acute intestinal bacterial infections and inflammatory bowel disease (IBD). METHODS: The quantitative expression of HLA-DR on monocytes was determined by fluorescence-activated cell sorting analysis in patients with IBD, patients with acute intestinal bacterial infections (bact.), and healthy subjects (contr.). RESULTS: The quantitative expression of HLA-DR in patients with bact. (n = 20; 90,000 molecules per monocyte; confidence interval [CI], 79,000-102,000) was significantly higher than that in patients with ulcerative colitis (n = 40, 30,000; CI, 30,000-38,000; P < 0.0001), Crohn disease (n = 80, 31,000; CI, 32,000-39,000; P < 0.0001), or in contr. (n = 28, 39,000; CI, 36,000-46,000; P < 0.0001). In patients with ulcerative colitis and Crohn disease, HLA-DR expression was significantly decreased, as compared with contr. (P < 0.05 and P < 0.01, respectively). With a cutoff point of 50,000, HLA-DR showed a sensitivity of 95% and a specificity of 92% in discriminating between bact. and active IBD. CONCLUSION: The quantitative measurement of HLA-DR expression could serve as a valuable tool to discriminate between bact. and active IBD.
Authors: Wolf-Dietrich Döcke; Conny Höflich; Kenneth A Davis; Karsten Röttgers; Christian Meisel; Paul Kiefer; Stefan U Weber; Monika Hedwig-Geissing; Ernst Kreuzfelder; Peter Tschentscher; Thomas Nebe; Andrea Engel; Guillaume Monneret; Andreas Spittler; Kathrin Schmolke; Petra Reinke; Hans-Dieter Volk; Dagmar Kunz Journal: Clin Chem Date: 2005-10-07 Impact factor: 8.327
Authors: Helmut Trimmel; Ursula Luschin; Karin Köhrer; Christian Anzur; Daniela Vevera; Andreas Spittler Journal: Shock Date: 2012-02 Impact factor: 3.454
Authors: M Ditschkowski; E Kreuzfelder; V Rebmann; S Ferencik; M Majetschak; E N Schmid; U Obertacke; H Hirche; U F Schade; H Grosse-Wilde Journal: Ann Surg Date: 1999-02 Impact factor: 12.969