| Literature DB >> 23860324 |
Zhujun Tan1, Shenglai Zhang, Maolan Li, Xiangsong Wu, Hao Weng, Qian Ding, Yang Cao, Runfa Bao, Yijun Shu, Jiasheng Mu, Qichen Ding, Wenguang Wu, Jiahua Yang, Lin Zhang, Yingbin Liu.
Abstract
Gallbladder carcinoma (GBC) is one of the mostly aggressive and fatal malignancies. However, little is known about the oncogenic genes that contributed to the development of GBC. Zinc finger X-chromosomal protein (ZFX) was a novel member of the Krueppel C2H2-type zinc-finger protein family and its down-regulation led to impaired cell growth in human laryngeal squamous cell carcinoma. Here, we aim to investigate the function of ZFX in GBC cell proliferation and migration. Loss of function analysis was performed on GBC cell line (GBC-SD) using lentivirus-mediated siRNA against ZFX. The proliferation, in vitro tumorigenesis (colony-formation) ability as well as cell migration was significantly suppressed after GBC-SD cells which were infected with ZFX-siRNA-expressing lentivirus (Lv-shZFX). Our finding suggested that ZFX promoted the growth and migration of GBC cells and could present a potential molecular target for gene therapy of GBC.Entities:
Keywords: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DMEM; Dulbecco's modified Eagle medium; EGFP; Enhanced green fluorescent protein; FBS; Fetal calf serum; GBC; Gallbladder carcinoma; HCC; Hepatocellular carcinoma; KCMF1; Lv-shZFX; MMP; MMP-2; MMP-9; MOI; MTT; Matrix metallopeptidase 2; Matrix metallopeptidase 9; Matrix metalloproteinase; Messenger RNA; Migration; Multiplicities of infection; PCR; Polymerase chain reaction; Potassium channel modulatory factor 1; Proliferation; Short hairpin RNAs; Small interfering RNA; ZBTB20; ZFX; ZFX-siRNA-expressing lentivirus; ZNF; ZNF267; Zinc finger; Zinc finger X-chromosomal protein; Zinc finger and BTB domain containing 20; Zinc finger protein 267; mRNA; shRNAs; siRNA
Mesh：
Substances：
Year: 2013 PMID： 23860324 DOI： 10.1016/j.gene.2013.06.064
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688