Light-evoked changes in extracellular calcium concentration in frog retina.

Light-evoked changes in extracellular Ca2+ concentration were recorded with Ca2(+)-selective microelectrodes in the retina of the frog eyecup. A Ca-decrease at light onset and offset was found in the inner plexiform layer, and its properties are consistent with it resulting from Ca2+ influx into activated neuronal terminals. In the subretinal space, a Ca-increase at light onset and a Ca-decrease at offset were observed, and these likely arise directly from photoreceptors. A slower ON Ca-decrease was also seen here. Because it survives pharmacological isolation of the photoreceptors from post-synaptic interactions, but not physical isolation of the retina from the pigment epithelium, this component probably depends on pigment epithelial activity.