| Literature DB >> 23852599 |
Andrea Stutz1, Gabor L Horvath, Brian G Monks, Eicke Latz.
Abstract
All inflammasomes require the adapter protein apoptosis associated speck-like protein containing a CARD (ASC) for the activation of caspase-1. After inflammasome activation, ASC assembles into a large protein complex, which is termed "speck". ASC specks can be observed as they reach a size of around 1 μm and in most cells only one speck forms upon inflammasome activation. Hence, ASC speck formation can be used as a simple upstream readout for inflammasome activation. Here, we describe a method for analyzing inflammasome activation by ASC speck visualization. First, we describe the generation of a clonal inflammasome reporter macrophage cell line overexpressing fluorescently tagged ASC. We then discuss stimulation conditions and the microscopic evaluation of ASC speck formation.Entities:
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Year: 2013 PMID： 23852599 DOI： 10.1007/978-1-62703-523-1_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745