An ultrasensitive electrochemical aptasensor with autonomous assembly of hemin-G-quadruplex DNAzyme nanowires for pseudo triple-enzyme cascade electrocatalytic amplification.

We for the first time fabricate a pseudo triple-enzyme cascade electrocatalytic electrochemical aptasensor by using the alcohol dehydrogenase (ADH) as well as the autonomously assembled hemin-G-quadruplex that simultaneously acted as an NADH oxidase and HRP-mimicking DNAzyme.