Karina Rodríguez-Capote1, Joceline Turner, Joseph Macri. 1. Department of Pathology and Laboratory Medicine, McMaster University, Ontario, Canada; Hamilton Regional Medicine Program, Ontario, Canada. Electronic address: karina.capote@dynalifedx.com.
Abstract
OBJECTIVE: The aim of this study was to evaluate the CEofix™ CDT Capillary Electrophoresis (CE) kit for the detection of β-2-tranferrin (β-2-Tf) in cerebrospinal fluid (CSF). DESIGN AND METHOD: Evaluation was performed according to CLSI EP5-A and EP12-A guidelines. RESULTS: The method resolved β-2-Tf from other Tf isoforms. The C50 for β-Tf was determined to be 0.46 mg/L. Neither hemoglobin nor bilirubin co-migrated with β-2-Tf. CONCLUSIONS: The CDT kit can be used for detecting β-2-Tf in CSF by CE.
OBJECTIVE: The aim of this study was to evaluate the CEofix™ CDT Capillary Electrophoresis (CE) kit for the detection of β-2-tranferrin (β-2-Tf) in cerebrospinal fluid (CSF). DESIGN AND METHOD: Evaluation was performed according to CLSI EP5-A and EP12-A guidelines. RESULTS: The method resolved β-2-Tf from other Tf isoforms. The C50 for β-Tf was determined to be 0.46 mg/L. Neither hemoglobin nor bilirubin co-migrated with β-2-Tf. CONCLUSIONS: The CDT kit can be used for detecting β-2-Tf in CSF by CE.