| Literature DB >> 23849778 |
Rim Hjeij1, Anna Lindstrand, Richard Francis, Maimoona A Zariwala, Xiaoqin Liu, You Li, Rama Damerla, Gerard W Dougherty, Marouan Abouhamed, Heike Olbrich, Niki T Loges, Petra Pennekamp, Erica E Davis, Claudia M B Carvalho, Davut Pehlivan, Claudius Werner, Johanna Raidt, Gabriele Köhler, Karsten Häffner, Miguel Reyes-Mugica, James R Lupski, Margaret W Leigh, Margaret Rosenfeld, Lucy C Morgan, Michael R Knowles, Cecilia W Lo, Nicholas Katsanis, Heymut Omran.
Abstract
The motive forces for ciliary movement are generated by large multiprotein complexes referred to as outer dynein arms (ODAs), which are preassembled in the cytoplasm prior to transport to the ciliary axonemal compartment. In humans, defects in structural components, docking complexes, or cytoplasmic assembly factors can cause primary ciliary dyskinesia (PCD), a disorder characterized by chronic airway disease and defects in laterality. By using combined high resolution copy-number variant and mutation analysis, we identified ARMC4 mutations in twelve PCD individuals whose cells showed reduced numbers of ODAs and severely impaired ciliary beating. Transient suppression in zebrafish and analysis of an ENU mouse mutant confirmed in both model organisms that ARMC4 is critical for left-right patterning. We demonstrate that ARMC4 is an axonemal protein that is necessary for proper targeting and anchoring of ODAs.Entities:
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Year: 2013 PMID： 23849778 PMCID： PMC3738828 DOI： 10.1016/j.ajhg.2013.06.009
Source DB: PubMed Journal: Am J Hum Genet ISSN： 0002-9297 Impact factor: 11.025