Two new steroids from an endophytic fungus Phomopsis sp.

Two new steroids, (14β,22E)-9,14-dihydroxyergosta-4,7,22-triene-3,6-dione (1) and (5α,6β,15β,22E)-6-ethoxy-5,15-dihydroxyergosta-7,22-dien-3-one (2), together with three known steroids, calvasterols A and B (3 and 4, resp.), and ganodermaside D (5), were isolated from the culture broth of an endophytic fungus Phomopsis sp. isolated from Aconitum carmichaeli. The structures of these compounds were elucidated on the basis of spectroscopic analysis, and their inhibitory activities against six pathogenic fungi were evaluated. Most of the compounds showed moderate or weak antifungal activities in a broth-microdilution assay.