| Literature DB >> 23845905 |
Qianqian Zheng1, Jian Gao, Honglin Li, Wendong Guo, Qi Mao, Enhui Gao, Ya-qin Zhu.
Abstract
Trefoil factor 3 (TFF3) is a member of the TFF-domain peptide family and essential in regulating cell migration and maintaining mucosal integrity in gastrointestinal tract. However, the role of TFF3 and its downstream regulating mechanisms in cancer cell migration remain unclear. We previously reported that TFF3 prolonged the up-regulation of Twist protein to modulate IL-8 secretion in intestinal epithelial cells. In this study, we investigated the role of Twist protein in TFF3-induced migration of SGC7901 cells. While Twist was activated by TFF3, siRNA-mediated knockdown of Twist abolished TFF3-induced cell migration. Furthermore, the migration related marker CK-8 as well as ZO-1 and MMP-9 was also regulated by TFF3 via a Twist-dependent mechanism. Our study suggests that Twist, as an important potential downstream effector, plays a key role in TFF3-modulated metastasis in gastric cancer and can be a promising therapeutic target against intestinal-type gastric cancer.Entities:
Keywords: CK-8; Gastric cancer; HRP; Horseradish peroxidase; ITF; Migration; TFF3; Trefoil factor 3; Twist; ZO-1; cytokeratin 8; intestinal trefoil factor; siRNA; small interfering RNA; trefoil factor 3; zonula occludens protein 1
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Year: 2013 PMID： 23845905 DOI： 10.1016/j.bbrc.2013.06.115
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575