PURPOSE: Massive small bowel resection (SBR) results in an adaptive response within the remnant bowel. We have previously shown an immediate reduction in intestinal blood flow and oxygen saturation (sO2) after SBR. We therefore sought to determine the duration of resection-induced intestinal hypoxia and expression of hypoxia-inducible factors (HIFs) following SBR. METHODS: C57B6 mice were subjected to 50% proximal SBR or a sham procedure. Photoacoustic microscopy (PAM) was used to measure blood flow and sO2 on postoperative days (PODs) 1, 3, and 7. Ileal tissue was harvested 6h postoperatively and on PODs 1 and 2, and HIF1α, HIF2α, and VEGF mRNA expression were assessed via RT-PCR. A p value of less than 0.05 was considered significant. RESULTS: Following SBR, reduction in intestinal blood flow persists for 24h and is followed with hyperemia by POD 3. The immediate reduction in venous sO2 and increased tissue oxygen utilization continued through POD 7. Enhanced expression of HIF1α was demonstrated 6h following SBR. CONCLUSION: Massive SBR results in an immediate relative hypoxic state within the remnant bowel with early enhanced expression of HIF1α. On POD 7, increased tissue oxygen extraction and elevated blood flow persist in the adapting intestine.
PURPOSE: Massive small bowel resection (SBR) results in an adaptive response within the remnant bowel. We have previously shown an immediate reduction in intestinal blood flow and oxygen saturation (sO2) after SBR. We therefore sought to determine the duration of resection-induced intestinal hypoxia and expression of hypoxia-inducible factors (HIFs) following SBR. METHODS: C57B6 mice were subjected to 50% proximal SBR or a sham procedure. Photoacoustic microscopy (PAM) was used to measure blood flow and sO2 on postoperative days (PODs) 1, 3, and 7. Ileal tissue was harvested 6h postoperatively and on PODs 1 and 2, and HIF1α, HIF2α, and VEGF mRNA expression were assessed via RT-PCR. A p value of less than 0.05 was considered significant. RESULTS: Following SBR, reduction in intestinal blood flow persists for 24h and is followed with hyperemia by POD 3. The immediate reduction in venous sO2 and increased tissue oxygen utilization continued through POD 7. Enhanced expression of HIF1α was demonstrated 6h following SBR. CONCLUSION: Massive SBR results in an immediate relative hypoxic state within the remnant bowel with early enhanced expression of HIF1α. On POD 7, increased tissue oxygen extraction and elevated blood flow persist in the adapting intestine.
Authors: Kolenkode B Kannan; Iriana Colorado; Diego Reino; David Palange; Qi Lu; Xiaofa Qin; Billy Abungu; Anthony Watkins; Francis J Caputo; Da-Zhong Xu; Gregg L Semenza; Edwin A Deitch; Rena Feinman Journal: Am J Physiol Gastrointest Liver Physiol Date: 2010-12-23 Impact factor: 4.052
Authors: Lucas A McDuffie; Brian T Bucher; Christopher R Erwin; Derek Wakeman; Francis V White; Brad W Warner Journal: J Pediatr Surg Date: 2011-06 Impact factor: 2.545
Authors: Murtaza M Tambuwala; Eoin P Cummins; Colin R Lenihan; Judit Kiss; Markus Stauch; Carsten C Scholz; Peter Fraisl; Felix Lasitschka; Martin Mollenhauer; Sean P Saunders; Patrick H Maxwell; Peter Carmeliet; Padraic G Fallon; Martin Schneider; Cormac T Taylor Journal: Gastroenterology Date: 2010-06-30 Impact factor: 22.682
Authors: Colin A Martin; Erin E Perrone; Shannon W Longshore; Paul Toste; Kathryn Bitter; Rajalakshmi Nair; Jun Guo; Christopher R Erwin; Brad W Warner Journal: J Pediatr Surg Date: 2009-06 Impact factor: 2.545
Authors: Kathryn J Rowland; Jose Diaz-Miron; Jun Guo; Christopher R Erwin; Junjie Mei; G Scott Worthen; Brad W Warner Journal: J Pediatr Surg Date: 2014-02-03 Impact factor: 2.545
Authors: Kristen M Seiler; Sarah E Waye; Wenjun Kong; Kenji Kamimoto; Adam Bajinting; William H Goo; Emily J Onufer; Cathleen Courtney; Jun Guo; Brad W Warner; Samantha A Morris Journal: Cell Mol Gastroenterol Hepatol Date: 2019-06-10
Authors: Emily J Onufer; Bola Aladegbami; Toru Imai; Kristen Seiler; Adam Bajinting; Cathleen Courtney; Stephanie Sutton; Aiza Bustos; Junjie Yao; Cheng-Hung Yeh; Anne Sescleifer; Lihong V Wang; Jun Guo; Brad W Warner Journal: PLoS One Date: 2020-09-15 Impact factor: 3.240