[Effect of lipopolysaccharide on proliferation and inflammatory factors expression of human periodontal ligament stem cells].

OBJECTIVE: To investigate the effect of Porphyromonas gingivalis lipopolysaccharide (LPS) on proliferation and inflammatory factors expression of human periodontal ligament stem cells (HPDLSCs).
METHODS: HPDLSCs were cultivated and identified. Experiment was divided into 3 groups according to culture solution: Group A with alpha-MEM culture solution containing 10 microg.mL-1 LPS, group B with supernatant fluid containing 10ng.mL-1 LPS stimulated monocyte, group C with alpha-MEM culture solution. The proliferation ability of HPDLSCs was analyzed by MTF assay. The expression levels of interleukin-1beta (IL-1beta), interleukin-6 (IL-6), tumor necrosis factor (TNF-alpha) mRNA of HPDLSCs were detected by reverse transcriptase polymerase chain reaction(RT-PCR).
RESULTS: HPDLSCs had clonality, bone and fat differentiation ability. Compared with group C, the proliferation ability of HPDLSCs of group A and group B was significantly inhibited, and the proliferation ability of HPDLSCs of group B were more significantly inhibited than that of group A (P<0.05). The expression of IL-1beta, IL-6 and TNF-alpha mRNA of group A and group B increased compared with the control group, and the expression of IL-1beta, IL-6 and TNF-alpha mRNA of group B increased more than that of group A (P<0.05).
CONCLUSION: Porphyromonas gingivalis may inhibit the proliferation of HPDLSCs directly or indirectly through LPS and increase expression of inflammatory factor, exacerbate periodontal inflammatory tissue damage and delay the self-repairing of periodontal tissue.