OBJECTIVE: To clarify the modulatory effects of daily consumption of pomegranate extract (PE), olive oil (OO) and Nagilla sativa oil (NSO) on antioxidant activity, sperm quality and pituitary-testicular axis of adult male wistar rats. METHODS: Thirty-two adult male Wistar rats were divided into four equal groups, eight rats each. Using rat gastric tubes, 1.0 mL distilled water, 1.0 mL PE, 0.4 mL NSO and 0.4 mL OO were orally administered daily for 6 weeks in the first, second, third and fourth groups, respectively. Reproductive organs, body weight, sperm criteria, testosterone, FSH, LH, inhibin-B, lipid peroxidation, and antioxidant enzyme activities were investigated. At the end of the study protocol, analyses occurred at the same time. Data were analysed by ANOVA test and P<0.05 was considered to be a significant value. RESULTS: In all studied groups, malondialdehyde level was significantly decreased accompanied with an increases in glutathione peroxidase and glutathione. Rats treated with PE showed an increase in catalase activities accompanied with an increase in sperm concentration which was also observed in NSO group. In PE treated group, sperm motility was also increased accompanied with decreased abnormal sperm rate. NSO, OO and PE treated groups shows an insignificant effect on testosterone, inhibin-B, FSH and LH in comparison with control group. CONCLUSIONS: These results show that administration of PE, NSO and OO could modify sperm characteristics and antioxidant activity of adult male wistar rats.
OBJECTIVE: To clarify the modulatory effects of daily consumption of pomegranate extract (PE), olive oil (OO) and Nagilla sativa oil (NSO) on antioxidant activity, sperm quality and pituitary-testicular axis of adult male wistar rats. METHODS: Thirty-two adult male Wistar rats were divided into four equal groups, eight rats each. Using rat gastric tubes, 1.0 mL distilled water, 1.0 mL PE, 0.4 mL NSO and 0.4 mL OO were orally administered daily for 6 weeks in the first, second, third and fourth groups, respectively. Reproductive organs, body weight, sperm criteria, testosterone, FSH, LH, inhibin-B, lipid peroxidation, and antioxidant enzyme activities were investigated. At the end of the study protocol, analyses occurred at the same time. Data were analysed by ANOVA test and P<0.05 was considered to be a significant value. RESULTS: In all studied groups, malondialdehyde level was significantly decreased accompanied with an increases in glutathione peroxidase and glutathione. Rats treated with PE showed an increase in catalase activities accompanied with an increase in sperm concentration which was also observed in NSO group. In PE treated group, sperm motility was also increased accompanied with decreased abnormal sperm rate. NSO, OO and PE treated groups shows an insignificant effect on testosterone, inhibin-B, FSH and LH in comparison with control group. CONCLUSIONS: These results show that administration of PE, NSO and OO could modify sperm characteristics and antioxidant activity of adult male wistar rats.
Authors: Filomena de Nigris; Sharon Williams-Ignarro; Lilach O Lerman; Ettore Crimi; Chiara Botti; Gelsomina Mansueto; Francesco P D'Armiento; Gaetano De Rosa; Vincenzo Sica; Louis J Ignarro; Claudio Napoli Journal: Proc Natl Acad Sci U S A Date: 2005-03-21 Impact factor: 11.205
Authors: Ahmet Ateşşahin; Gaffari Türk; Seval Yilmaz; Mustafa Sönmez; Fatih Sakin; Ali Osman Ceribasi Journal: Basic Clin Pharmacol Toxicol Date: 2010-01-14 Impact factor: 4.080
Authors: Maja D K Fedder; Henrik B Jakobsen; Ina Giversen; Lars P Christensen; Erik T Parner; Jens Fedder Journal: PLoS One Date: 2014-10-02 Impact factor: 3.240
Authors: Fatma M Minisy; Hossam H Shawki; Abdelfatteh El Omri; Ahmed A Massoud; Enayat A Omara; Fatma G Metwally; Manal A Badawy; Neveen A Hassan; Nabila S Hassan; Hisashi Oishi Journal: Biomed Res Int Date: 2020-03-09 Impact factor: 3.411