OBJECTIVE: This study examined the effect of basic fibroblast growth factor (FGF)-2 with an absorbable collagen sponge on angiogenesis and bone regeneration in rat calvarial critical-sized bone defects using microcomputed tomography. METHODS: Forty 7-week-old male Fischer rats were used. The symmetrical critical-sized calvarial bone defects (5 mm diameter) were created. An absorbable collagen sponge with or without FGF-2 (0.1% or 0.3%) was implanted into each bone defect. RESULTS: Blood vessel and bone volumes were significantly higher in the 0.3% FGF-2 group compared with the control and 0.1% FGF-2 groups on day 28. Significantly more osteoblast- and osteoclast-like cells were seen in the 0.3% FGF-2 group. CONCLUSIONS: Thus, FGF-2 increased blood vessel and bone formation in rat calvarial critical-sized bone defects.
OBJECTIVE: This study examined the effect of basic fibroblast growth factor (FGF)-2 with an absorbable collagen sponge on angiogenesis and bone regeneration in rat calvarial critical-sized bone defects using microcomputed tomography. METHODS: Forty 7-week-old male Fischer rats were used. The symmetrical critical-sized calvarial bone defects (5 mm diameter) were created. An absorbable collagen sponge with or without FGF-2 (0.1% or 0.3%) was implanted into each bone defect. RESULTS: Blood vessel and bone volumes were significantly higher in the 0.3% FGF-2 group compared with the control and 0.1% FGF-2 groups on day 28. Significantly more osteoblast- and osteoclast-like cells were seen in the 0.3% FGF-2 group. CONCLUSIONS: Thus, FGF-2 increased blood vessel and bone formation in rat calvarial critical-sized bone defects.
Authors: Patricia Carlisle; Jeffrey Marrs; Laura Gaviria; David T Silliman; John F Decker; Pamela Brown Baer; Teja Guda Journal: Tissue Eng Part C Methods Date: 2019-12 Impact factor: 3.056