| Literature DB >> 23831060 |
Ana M Rodríguez-Torres1, Mónica Lamas-Maceiras, Rosa García-Díaz, María A Freire-Picos.
Abstract
The eukaryotic Ssu72 factor is involved in several RNA biogenesis processes. It has phosphatase activity on the carboxy-terminal domain (CTD) of the major subunit of RNA polymerase II. The Kluyveromyces lactis Ssu72 (KlSsu72) shows in vitro phosphatase activity for the pNPP substrate, and this activity is inhibited by ortho-vanadate. The expression of KlSsu72 in Saccharomyces cerevisiae shows defective CTD serine5-P phosphatase activity and reveals the importance of Ssu72 for the normal CTD serine5-P levels at two growth states. The divergence is emphasised by the remarkable changes in RNA14 alternative 3'-end RNA processing, which are independent of the CTD serine5-P levels.Entities:
Keywords: 5-fluoro-orotic acid; CTD; CTD-phosphatase; FOA; GST; OD(600); RNA polymerase II; RNA polymerase-II; RNA-processing; RNAPII; SSU72; Ser2, Ser5; Yeast; carboxy-terminal domain (CTD) of the major subunit of RNA polymerase II; glutathione S-transferase; optical density at 600 nanometers; pNPP; paranitrophenylphosphate; serine 2 and 5, respectively, from CTD repeats
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Year: 2013 PMID： 23831060 DOI： 10.1016/j.febslet.2013.06.044
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124