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Literature DB >> 2383010

Isolation of a single-stranded plasmid from Clostridium acetobutylicum NCIB 6444.

Abstract

The cryptic plasmid pDM6 was isolated from late exponential-phase cells of Clostridium acetobutylicum NCIB 6444 by either alkaline lysis or electroporation. The application of high voltage during electroporation resulted in higher DNA yield than did the alkaline lysis procedure. However, electroporation-induced plasmid release generated high amounts of single-stranded DNA compared with the alkaline lysis procedure, which generated both double-stranded DNA (monomer and dimer forms) and single-stranded DNA.

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Year: 1990 PMID： 2383010 PMCID： PMC184500 DOI： 10.1128/aem.56.6.1725-1728.1990

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

13 in total

1. Transformation of Heat-Treated Clostridium acetobutylicum Protoplasts with pUB110 Plasmid DNA.

Authors: Y L Lin; H P Blaschek
Journal: Appl Environ Microbiol Date: 1984-10 Impact factor: 4.792

Review 2. Recent advances in the genetics of the clostridia.

Authors: M Young; N P Minton; W L Staudenbauer
Journal: FEMS Microbiol Rev Date: 1989-12 Impact factor: 16.408

3. Extracellular nucleases of pseudomonas BAL 31. III. Use of the double-strand deoxyriboexonuclease activity as the basis of a convenient method for the mapping of fragments of DNA produced by cleavage with restriction enzymes.

Authors: R J Legerski; J L Hodnett; H B Gray
Journal: Nucleic Acids Res Date: 1978-05 Impact factor: 16.971

2. Heterologous expression of endo-beta-1,4-D-glucanase from Clostridium cellulovorans in Clostridium acetobutylicum ATCC 824 following transformation of the engB gene.

Authors: A Y Kim; G T Attwood; S M Holt; B A White; H P Blaschek
Journal: Appl Environ Microbiol Date: 1994-01 Impact factor: 4.792

3. Construction and characterization of a phage-plasmid hybrid (phagemid), pCAK1, containing the replicative form of viruslike particle CAK1 isolated from Clostridium acetobutylicum NCIB 6444.

Authors: A Y Kim; H P Blaschek
Journal: J Bacteriol Date: 1993-06 Impact factor: 3.490

3 in total

Isolation of a single-stranded plasmid from Clostridium acetobutylicum NCIB 6444.

1. Transformation of Heat-Treated Clostridium acetobutylicum Protoplasts with pUB110 Plasmid DNA.

Review 2. Recent advances in the genetics of the clostridia.

3. Extracellular nucleases of pseudomonas BAL 31. III. Use of the double-strand deoxyriboexonuclease activity as the basis of a convenient method for the mapping of fragments of DNA produced by cleavage with restriction enzymes.

4. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules.

5. Oxygen and the growth and metabolism of Clostridium acetobutylicum.

6. High-efficiency transformation of bacterial cells by electroporation.

7. Electroporation-induced transformation of intact cells of Clostridium perfringens.

8. Plasmid detection and isolation in strains of Clostridium acetobutylicum and related species.

9. Plasmid structural instability associated with pC194 replication functions.

10. Development of a new shuttle plasmid system for Escherichia coli and Clostridium perfringens.

1. Isolation and characterization of a filamentous viruslike particle from Clostridium acetobutylicum NCIB 6444.

2. Heterologous expression of endo-beta-1,4-D-glucanase from Clostridium cellulovorans in Clostridium acetobutylicum ATCC 824 following transformation of the engB gene.

3. Construction and characterization of a phage-plasmid hybrid (phagemid), pCAK1, containing the replicative form of viruslike particle CAK1 isolated from Clostridium acetobutylicum NCIB 6444.