BACKGROUND: The European Bioanalysis Forum dried blood spots (DBS)/microsampling consortium is reporting back from the experiments they performed on further documenting the potential hurdles of the DBS technology. This paper is focused on the impact of hematocrit changes on DBS analyses. RESULTS: The hematocrit can have an effect on the size of the blood spot, on spot homogeneity and on extraction recovery in a compound-dependent manner. The extraction recovery can change upon aging in an hematocrit-dependent way. Different card materials can give different outcomes. CONCLUSIONS: The results from the conducted experiments show that the issues of DBS in regulated bioanalysis are real and that the technology will need improvements to be ready for use as a general tool for regulated bioanalysis.
BACKGROUND: The European Bioanalysis Forum dried blood spots (DBS)/microsampling consortium is reporting back from the experiments they performed on further documenting the potential hurdles of the DBS technology. This paper is focused on the impact of hematocrit changes on DBS analyses. RESULTS: The hematocrit can have an effect on the size of the blood spot, on spot homogeneity and on extraction recovery in a compound-dependent manner. The extraction recovery can change upon aging in an hematocrit-dependent way. Different card materials can give different outcomes. CONCLUSIONS: The results from the conducted experiments show that the issues of DBS in regulated bioanalysis are real and that the technology will need improvements to be ready for use as a general tool for regulated bioanalysis.
Authors: Christopher Evans; Mark Arnold; Peter Bryan; Jeffrey Duggan; Christopher A James; Wenkui Li; Steve Lowes; Luca Matassa; Timothy Olah; Philip Timmerman; Xiaomin Wang; Enaksha Wickremsinhe; John Williams; Eric Woolf; Patricia Zane Journal: AAPS J Date: 2014-12-09 Impact factor: 4.009
Authors: Daniel Gonzalez; Chiara Melloni; Brenda B Poindexter; Ram Yogev; Andrew M Atz; Janice E Sullivan; Susan R Mendley; Paula Delmore; Amy Delinsky; Kanecia Zimmerman; Andrew Lewandowski; Barrie Harper; Kenneth C Lewis; Daniel K Benjamin; Michael Cohen-Wolkowiez Journal: Bioanalysis Date: 2015 Impact factor: 2.695
Authors: A E C A B Willemsen; L M Knapen; Y M de Beer; R J M Brüggemann; S Croes; C M L van Herpen; N P van Erp Journal: Eur J Clin Pharmacol Date: 2017-12-08 Impact factor: 2.953
Authors: Beatriz Uribe; Oskar González; María Encarnación Blanco; Oihane Elena Albóniga; María Luz Alonso; Rosa María Alonso Journal: Molecules Date: 2019-11-26 Impact factor: 4.411