| Literature DB >> 23827816 |
Pedro M F Sousa1, Marco A M Videira, Ana M P Melo.
Abstract
The Escherichia coli formate:oxygen oxidoreductase supercomplex (FdOx) was investigated with respect to function and composition. Formate oxidoreductase activity was detected in blue native polyacrylamide gel electrophoresis (BN-PAGE) resolved membranes of E. coli, which were also capable of cyanide sensitive formate:oxygen oxidoreductase activity. The latter was compromised in strains devoid of specific oxygen reductases, particularly, in those devoid of cytochrome bo3 or bdI. A principal component analysis (PCA) integrating E. coli aerobic respiratory chain gene transcription, enzyme activity and growth dynamics was performed, correlating formate:oxygen oxidoreductase activity and the transcription of the genes encoding cytochromes bo3 and bdI, and corroborating previous evidence that associated these complexes in FdOx.Entities:
Keywords: 3-(N-Morpholino) propanesulfonic acid; AU; Aerobic respiratory chain supercomplex; BN-PAGE; Bacteria; Blue native polyacrylamide gel electrophoresis; DCPIP; DDM; Escherichia coli; FDH; FdOx; Formate:oxygen oxidoreductase activity; Gene transcription; K(3)[Fe(CN)(6)]; K(M); MOPS; Michaelis Menten constant; NADH:quinone oxidoreductase; NBT; NDH-1; NDH-2; OR; Oxygen reductase; PCA; PMS; UQ(1); V(MAX); alternative NADH:quinone oxidoreductase; arbitrary units; blue native polyacrylamide gel electrophoresis; coenzyme Q1, analogue to ubiquinone; deNADH; deamino-NADH; dichlorophenol indophenol; dodecyl-β-d-maltoside; formate dehydrogenase; formate:oxygen oxidoreductase supercomplex; maximal velocity; nitroblue tetrazolium; oxidoreductase; phenazine methosulfate; potassium ferricyanide; principal component analysis
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Year: 2013 PMID： 23827816 DOI： 10.1016/j.febslet.2013.06.031
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124