AIM: To analyze proteomic and signal transduction alterations in irradiated melanoma cells. METHODS: We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. Protein-protein interaction was used to analyze relationships among proteins. RESULTS: Energy metabolism protein levels were significantly different in glycolysis and not significantly different in oxidative phosphorylation after irradiation. Conversely, tumor suppressor proteins related to cell growth and development were downregulated, and those related to cell death and cell cycle were upregulated in irradiated cells. CONCLUSION: Our results indicate that irradiation induces differential expression of the 29 identified proteins closely related to cell survival, cell cycle arrest, and growth inhibition. The data may provide new insights into the pathogenesis of uveal melanoma and guide appropriate radiotherapy.
AIM: To analyze proteomic and signal transduction alterations in irradiated melanoma cells. METHODS: We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. Protein-protein interaction was used to analyze relationships among proteins. RESULTS: Energy metabolism protein levels were significantly different in glycolysis and not significantly different in oxidative phosphorylation after irradiation. Conversely, tumor suppressor proteins related to cell growth and development were downregulated, and those related to cell death and cell cycle were upregulated in irradiated cells. CONCLUSION: Our results indicate that irradiation induces differential expression of the 29 identified proteins closely related to cell survival, cell cycle arrest, and growth inhibition. The data may provide new insights into the pathogenesis of uveal melanoma and guide appropriate radiotherapy.
Authors: Thomas Santarius; Graham R Bignell; Chris D Greenman; Sara Widaa; Lina Chen; Claire L Mahoney; Adam Butler; Sarah Edkins; Sahar Waris; Paul J Thornalley; P Andrew Futreal; Michael R Stratton Journal: Genes Chromosomes Cancer Date: 2010-08 Impact factor: 5.006
Authors: M Schuhmacher; F Kohlhuber; M Hölzel; C Kaiser; H Burtscher; M Jarsch; G W Bornkamm; G Laux; A Polack; U H Weidle; D Eick Journal: Nucleic Acids Res Date: 2001-01-15 Impact factor: 16.971
Authors: Manuel F Bande Rodríguez; Beatriz Fernandez Marta; Nerea Lago Baameiro; Maria Santiago-Varela; Paula Silva-Rodríguez; María Jose Blanco-Teijeiro; Maria Pardo Perez; Antonio Piñeiro Ces Journal: Clin Ophthalmol Date: 2020-01-20
Authors: John W Crabb; Bo Hu; John S Crabb; Pierre Triozzi; Yogen Saunthararajah; Raymond Tubbs; Arun D Singh Journal: PLoS One Date: 2015-08-25 Impact factor: 3.240