Literature DB >> 23825800

Microplate-compatible total internal reflection fluorescence microscopy for receptor pharmacology.

Minghan Chen1, Natalya V Zaytseva, Qi Wu, Min Li, Ye Fang.   

Abstract

We report the use of total internal reflection fluorescence (TIRF) microscopy for analyzing receptor pharmacology and the development of a microplate-compatible TIRF imaging system. Using stably expressed green fluorescence protein tagged β2-adrenergic receptor as the reporter, we found that the activation of different receptors results in distinct kinetic signatures of the TIRF intensity of cells. These TIRF signatures closely resemble the characteristics of their respective label-free dynamic mass redistribution signals in the same cells. This suggests that TIRF in microplate can be used for profiling and screening drugs.

Entities:  

Year:  2013        PMID： 23825800      PMCID： PMC3669112          DOI： 10.1063/1.4805041

Source DB:  PubMed          Journal:  Appl Phys Lett        ISSN： 0003-6951            Impact factor:   3.791


  21 in total

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