| Literature DB >> 23818666 |
Smrutisanjita Behera1, Jörg Kudla.
Abstract
This protocol describes a method for imaging cytoplasmic Ca(2+) dynamics in roots with high resolution using confocal laser scanning microscopy (CLSM). The Förster (fluorescence) resonance energy transfer (FRET)-based genetically modified Ca(2+) indicator Yellow Cameleon YC3.6, stably expressed in plants under the control of the ubiquitin promoter UBQ10, is used for Ca(2+) measurements. This protocol enables imaging of 5- to 7-d-old seedlings with high-magnification objectives (25×, 40×, and 63×).Entities:
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Year: 2013 PMID： 23818666 DOI： 10.1101/pdb.prot073023
Source DB: PubMed Journal: Cold Spring Harb Protoc ISSN： 1559-6095