| Literature DB >> 23816690 |
Mingning Qiu1, Fusheng Quan, Chengquan Han, Bin Wu, Jun Liu, Zhongcai Yang, Feng Su, Yong Zhang.
Abstract
The aim of this study was to investigate the effect of granulosa cells from small antral follicles on steroidogenesis, proliferation and apoptosis of goat ovarian stromal and theca cells in vitro. Using Transwell co-culture system, we evaluated androgen production, LH responsiveness, cell proliferation and apoptosis and some molecular expression regarding steroidogenic enzyme and apoptosis-related genes in stromal and theca cells. The results indicated that the co-culture with granulosa cells increased steroidogenesis, LH responsiveness and bcl-2 gene expression as well as decreased apoptotic bax and bad expressions in stromal and theca cells. Thus, granulosa cells had a capacity of promoting steroidogenesis in stromal cell and LH responsiveness in cortical stromal cells, maintaining steroidogenesis in theca cells, inhibiting apoptosis of cortical stromal cells and improving anti-apoptotic abilities of stromal and theca cells.Entities:
Keywords: 17alpha-hydroxylase/C17, 20-lyase; 3beta-hydroxysteroid deydrogenase/isomerase; Apoptosis; B-cell lymphoma protein 2; BCL-2-associated X protein; BCL-2-associated agonist of cell death; Bad; Bax; Bcl-2; CYP11A1; CYP17A1; Cell proliferation; Cholesterol side-chain cleavage cytochrome P450; GAPDH; GCs; Goat; Granulosa cells; HSD3B1; LHR; Ovarian stromal cells; SCs; SMs; STAR; Steroidogenesis; TCs; Theca cells; glyceraldehyde-3-phosphate dehydrogenase; granulosa cells; luteinizing hormone receptor; ovarian cortical stromal cells; ovarian medullary stromal cells; steroidogenic acute regulatory protein; theca cells
Mesh：
Year: 2013 PMID： 23816690 DOI： 10.1016/j.jsbmb.2013.06.005
Source DB: PubMed Journal: J Steroid Biochem Mol Biol ISSN： 0960-0760 Impact factor: 4.292