| Literature DB >> 23816433 |
Meichun Zhang1, Xiaoying Zheng, Yu Wu, Ming Gan, Ai He, Zhuoya Li, Dongjing Zhang, Xiansheng Wu, Ximei Zhan.
Abstract
The interaction between dengue virus (DENV) and vector mosquitoes are still poorly understood at present. In this study, 2-D DIGE combined with MS was used to analyze the differential proteomes of Aedes albopictus salivary gland, midgut and C6/36 cells induced by DENV-2. Our results indicated that the virus infection regulated several functional classes of proteins. Among them, 26 were successfully analyzed by real-time RT-PCR. The mRNA levels of 15 were the highest in salivary gland, 2 in midgut and none in C6/36 cells, however, 18 were the least in fat body compared to other organs. Interestingly, the changes of differential proteins mRNA were the most obvious in fat body post-infection. Chaperone, cytoskeleton and energy metabolism enzyme were the most down- or up- regulated proteins after DENV-2 infection. The abundant expression of these proteins in salivary gland may relate to its high susceptibility.Entities:
Keywords: Aedes albopictus; Dengue virus; Differential proteomics
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Year: 2013 PMID： 23816433 DOI： 10.1016/j.virol.2013.06.001
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616